Discovery® DPA-6S SPE Tube

bed wt. 1 g, volume 12 mL, pk of 20



PE frit (20 μm)

Quality Level


bed wt., 1 g


pk of 20


12 mL


<5% Water content


polyamide resin base material

matrix active group

amide, poly- phase

particle size

50-160 μm

pH range

4.5 - 7.5 (surface pH)

bulk density

0.2‑0.3 g/mL

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous or methanolic solutions

  • Polyamide Resin: Particle Size: 50-160μm, Surf pH: 4.5-7.5, Density: 0.2-0.3 cm3/g, Water Content: < 5 %
  • Used to adsorb polar compounds (-OH groups, esp. phenolic compounds) from aqueous or methanolic solutions under the reversed-phase mechanism through strong hydrogen bonding between compound hydroxyl groups and amide groups of the resin
  • Useful for extracting tannins, chlorophyll, humic acid, pharmacologically active terpenoids, flavanoids, gallic acid, catechol A, protocatechuic acid, and phloroglucinol
  • Also useful for extracting aromatic carboxylic acids and nitroaromatic compounds
  • Irreversibly retains quinones

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC
Certificado de Análisis
Takayuki Asada et al.
Journal of chromatography. A, 1351, 21-28 (2014-06-04)
Isolation techniques for anthocyanidin 3,5-diglucosides and their derivatives from three types of supramolecular pigments were newly developed using two SPE (Discovery DPA-6S and DSC-SCX) cartridges. Initially, malonylawobanin from commelinin supramolecular complex yielded 79.5% with 85.7% purity using DPA-6S, while DSC-SCX...
Takayuki Asada et al.
Food chemistry, 166, 10-16 (2014-07-24)
An aluminium complex of crude Delaware grape anthocyanins, bearing an ortho-dihydroxyl group on the B ring and/or a p-coumaroyl group as an ester with flavocommelin, was formed and isolated by ethanol precipitation. Using a Discovery DPA-6S short column, selected anthocyanins...
Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.
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