., ed., Practical Handbook of
Biochemistry and Molecular Biology, CRC Press,
(1986), p. 464.
MAM 6/01
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
., Ann. Rev. Biochem., 50, 433-
464 (1981)
4. Borsos, T., et al., J. Immunol., 105, 1439 (1970)
5. Martin, A., et al., Immunochemistry, 13, 317-324
(1976)
6. Thomas, A.E., et al., J. Immunology
stable
for at least 6 months when stored appropriately.
Product Profile
A recommended working concentration of 0.05 to
1.0 µg/ml is determined by dot blot. In ELISA a 1:200
working dilution is
mass
spectrometry. SILuProt CLU is a heterodimer of
2 subunits (alpha and beta) consisting of 464 amino
acids (including N-terminal polyhistidine and V5 tags),
with a calculated molecular mass of
Protein Eng. Des. Sel.,
23(11), 843-848 (2010).
16. Ritchie, T.K., et al., Methods Enzymol., 464, 211-
231 (2009).
17. Supplier information.
For a further and extensive list of citations and
Protein Eng. Des. Sel.,
23(11), 843-848 (2010).
16. Ritchie, T.K., et al., Methods Enzymol., 464, 211-
231 (2009).
17. Supplier information.
For a further and extensive list of citations and
Temperature
1 Pipette 200 �l of standard dilutions,
or 200 �l of samples into MP wells
200 �l 1 h at +37°C
2 Wash 5 times with washing buffer (solution 6) 5 × 250 �l 5 × 30 s
Protein Eng. Des. Sel.,
23(11), 843-848 (2010).
16. Ritchie, T.K., et al., Methods Enzymol., 464, 211-
231 (2009).
17. Siuda, I., and Tieleman, D.P., J. Chem. Theory
Comput., 11(10), 4923-
T.H., et al., Nano Letters, 2(8), 853-856
(2002).
15. Ritchie, T.K., et al., Methods Enzymol., 464, 211-
231 (2009).
16. Supplier information.
For a further and extensive list of citations and
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
TD,MAM 07/11-1
Sigma
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
PRECISIO is a registered trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich
CDK2 ∼58 kDa
CyclinE1 ∼73 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 343–464 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
JR,JB,MAM 10/12-1
2012
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
PRECISIO is a registered trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich
CDK2 ∼58 kDa
CyclinE1 ∼73 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 343–464 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
CDK2 ∼58 kDa
CyclinE1 ∼73 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 343–464 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
CDK2 ∼58 kDa
CyclinE1 ∼73 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 343–464 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
Precisio is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co.
TLD
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
Precisio is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co.
TLD
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
Precisio is a registered trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich
The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
TLD,MAM 02/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich