non sterile SLFH025NS
Fig. 2: Developed plate at 254 nm
Eusolex® 9020
M = 310.4 g/mol [M+H]+
[M+Na]+
Eusolex® 9020 standard
Sun Cream 1
Sun Cream 2
1 2 3 4 5 6 7
GelGreen® has UV absorption between 250 nm and 300 nm and a
strong absorption peak centered around 500 nm (Figure 1). Thus,
GelGreen® is compatible with either a 254 nm UV transilluminator or a
gel reader
GelGreen® has UV absorption between 250 nm and 300 nm and a
strong absorption peak centered around 500 nm (Figure 1). Thus,
GelGreen® is compatible with either a 254 nm UV transilluminator or a
gel reader
30,000 µJoules/cm2 at 254 nm.
Optimization of UV Cross-linking Energy.
Recommendation: For maximum hybridization signal, cross-link either with 20,000 µJoules/cm2 at 254 nm for DNA probes or
Supelco SPB-1 (Cat. No. 2-4162)
I.D. 0.20 mm Length 15 m Film Thickness 0.20 µm
Oven Temperature 50°C (2 min.), then 15°/min. to 300°C,
hold 11 min.
Injector Temperature 250°C, Split
Detector Hewlett-Packard
Cronan, J. E., Jr., Re-evaluation
of the solution structure of acyl carrier protein. J.
Biol. Chem., 254, 9778-9785 (1979).
6. Rock, C. O., et al., Molecular properties of acyl
carrier protein derivatives
was used at a flow rate of 1.0 ml per minute. The
product was detected by measuring absorbance at
254 nm and comparison was made to a standard curve
for 6β-hydroxytestosterone (Product No. H 2898).
■ 5,000 µJoules/cm2 at 254 nm for direct spotting of bacterial suspensions on a
membrane surface without further culture.
■ 60,000 µJoules/cm2 at 254 nm for 2 mm diameter colonies.
■
Characterization, Academic
Press, (New York, NY: 1993) pp. 34-45.
8. Blackburn, P., J. Biol. Chem., 254, 12484 (1979).
9. de Martynoff, G. et al., Biochem. Biophys. Res.
Commun., 93, 645 (1980).
10.
Molecular mass: ∼63 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 188–254 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for drug,
household
Page 2 of 4
Enzymatic Assay of ADP-RIBOSYLCYCLASE
REAGENTS: (continued)
E. 250 J�PO�&\FOLF�$GHQRVLQH�Diphosphate-Ribose Standard Solution (cADP Ribose)
(Prepare 1 ml by dissolving a 250 J�YLDO
interleukin-
1band nerve growth factor in the striatum.
Neurosci. Lett., 250, 25-28 (1998).
12. Teismann, P., et al., Cyclooxygenase-2 is
instrumental in Parkinson's disease
neurodegeneration. Proc. Natl
30(3),
188-191 (2000).
7. Jacobs, N.J., and VanDemark, P.J., Arch. Biochem.
Biophys., 88(2), 250-255 (1960).
Triton is a trademark of The Dow Chemical Company or
an affiliated company of Dow
enzyme
at –70 °C. Store the enterokinase buffer at 2–8 °C.
Procedure
Optimal results have been obtained with FLAG fusion
protein concentrations at ∼250 ng/ml. The pH of the
FLAG fusion protein solution
reducing agents on
the recovery of injured Bifidobacterium cells.
J. Microbiol. Methods, 49(3), 247-254 (2002).
6. Melder, R. J., and Jain, R. K., Reduction of rigidity
in human activated natural
Characterization, Academic
Press, (New York, NY: 1993) pp. 34-45.
8. Blackburn, P., J. Biol. Chem., 254, 12484 (1979).
9. de Martynoff, G. et al., Biochem. Biophys. Res.
Commun., 93, 645 (1980).
10.
-β
and nerve growth factor in the striatum. Neurosci.
Lett., 250, 25-28 (1998).
12. Teismann, P., et al., Cyclooxygenase-2 is
instrumental in Parkinson's disease
neurodegeneration. Proc
properties of L-alpha-glycerophosphate oxidase
from Streptococcus faecium ATCC 12755. J. Biol.
Chem., 254, 2710-15 (1979).
4. Finnerty, C.M., et al., Crystallization and preliminary
crystallographic analysis
carboxypeptidase A. J. Biol.
Chem., 250, 3831-3836 (1975).
6. Love, J.D., and Hewitt, R.R., The relationship
between human serum and human pancreatic
DNase I. J. Biol. Chem., 254, 12588-12594 (1979).
DT
2,4,6-trimethylphenyl)amine]
Catalog Number 702471
Store at Room Temperature
Technical Bulletin AL-254
TECHNICAL BULLETIN
Synonyms: Poly(triaryl amine), PTAA
Product Description
[C6H4N(C6H2(
References
1. Uchida, Y. et al., J. Biochem., 86(5), 1573-1585
(1979).
2. Supplier data
3. Saito, N. et al., J. Biol. Chem., 254(16), 7845-7854
(1979).
Trizma is a registered trademark of Sigma-Aldrich
carboxypeptidase A. J. Biol.
Chem., 250, 3831-3836 (1975).
6. Love, J.D., and Hewitt, R.R., The relationship
between human serum and human pancreatic DNase I.
J. Biol. Chem., 254, 12588-12594 (1979).
DT,RC
(1991).
2. Vlamis-Gardikas, A., et al., J. Biol. Chem., 272,
11236-11243 (1997).
3. Aslund, F., et al., Proc. Natl. Acad., USA, 91, 9813-
9817 (1994).
4. Holmgren, A., J Biol. Chem., 254, 3664-3671