Avoid freeze-thaw cycles. Store
working aliquots at –70 °C. The product is stable for
24 to 48 hours at 2-8 °C.
References
1. Cohen, P., et al., Protein phosphatase-1 and
protein phosphatase-2A
Inhibition of human dihydrofolate reductase by antifolyl polyglutamates.
Biochem. Pharm. 38, 541-543 (1989)
19. Sharif, K.A., et al., Concentrating capacity of the human reduced folate carrier (hRFC1
hydroxy-2-
nonenal from the autoxidation of polyunsaturated
fatty acids. Free Radic. Biol. Med., 8, 541-543
(1990).
2. Esterbauer, H., et al., Chemistry and biochemistry
of 4-hydroxy-2-nonenal, maloaldehyde
Inhibition of human
dihydrofolate reductase by antifolyl
polyglutamates. Biochem. Pharm. 38, 541-
543 (1989)
19. Sharif, K.A., et al., Concentrating capacity
of the human reduced folate carrier
and splenocytes from
BALB/c mice immunized with a synthetic peptide
corresponding to amino acids 543-555 of human JAK1,
conjugated to KLH. The peptide sequence is identical in
mouse and rat JAK1. The
diphenylcarbazone to give a red-violet
color complex, which is measured photometrically at or
near 543 nm. The reaction producing the red-violet color
only occurs in the presence of Cr6+, i.e. chromate
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM potassium phosphate containing 1.0 M NaCl, pH 7.8, for
approx. 24 hours before use, using a flow rate of 2 mL
Cytokine, 8, 864 (1996).
5. Van der Poll, T., et al., Blood, 86, 2754 (1995).
6. Krown, K. A., et al., FEBS Lett., 376, 24 (1995).
7. Paleolog, E. M., et al., Blood, 84, 2578 (1994).
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM potassium phosphate containing 1.0 M NaCl, pH 7.8, for
approx. 24 hours before use, using a flow rate of 2 mL
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM potassium phosphate containing 1.0 M NaCl, pH 7.8, for
approx. 24 hours before use, using a flow rate of 2 mL
diphenylcarbazone to give a red-
violet color complex that is measured photometrically or near at 543 nm. The reaction,
producing the red violet color, only occurs in the presence of Cr6+, i.e. chromate
incubate overnight (20-24 hours) at 4°C.
B. Day Two
6. Pipet 100 µL of 125I-Human Leptin to all tubes.
7. Vortex, cover, and incubate overnight (20-24 hours) at 4°C.
C. Day Three
8. Add 1.0 mL
NSB tubes
(3-4).
6. Vortex, cover, and incubate overnight (20-24 hours) at 4°C.
HL-81K-Rev 10-MAY-2017 Millipore 8
VIII. ASSAY PROCEDURE (continued)
B. Day Two
Status (Stanley, Kricka, eds.) J. Wiley & Sons,
New York, p. 543.
23 Ford, S. R., Hall, M. S. & Leach, F. R. (1992) Anal. Biochem. 204, 283.
24 Brasier A. R. (1990) In: Current Protocols in Molecular Biology