manténgalo a menos de 60 °C.
• Retire la tapa esmerilada de el casete. 1
• Utilice una jeringa o pipeta de dispen-
sación para añadir 9,5-10 ml de agar
en el centro de el casete. 2
Z748307 Large 1 L load flask and bulb-tube, one straight tube, 24/40 joints
Z748315 X-Large 2 L load flask and bulb-tube, one straight tube, 24/40 joints
Kugelrohr Load Flasks
Robust glass rod enables
diameter
(mm)
Growth
area (cm2)
Factor to 24-
wells
Media volume
(ml)
DNA/ESCORT V
cocktail to add
100 mm 100.00 78.50 41.00 21.00 3 - 8 ml
60 mm 60.00 30.00 14.70 8.00 1 -
steps 2-4 every day for a total of 4 days.
Day 8: Quality Control of Hindgut differentiation.
6. Set up flow analysis for CDX-2, a hindgut endoderm marker. Expression of CDX-2 must be >
Gebrauchsanweisung:
1. Teststäbchen 1s mit beiden Testfeldern in Probe (pH 1–9) eintauchen.
2. Flüssigkeitsüberschuss abschütteln.
3. 60 s warten.
4. Teststäbchen mit Farbskala vergleichen. Sind Nitrat-Ionen vorhanden
Gebrauchsanweisung:
1. Teststäbchen 1s mit beiden Testfeldern in Probe (pH 1–9) eintauchen.
2. Flüssigkeitsüberschuss abschütteln.
3. 60 s warten.
4. Teststäbchen mit Farbskala vergleichen. Sind Nitrat-Ionen vorhanden
PBS 48 mL
Catalog Number MAK194A
Formalin (10%) 24 mL
Catalog Number MAK194B
Oil Red O 60 mg
Catalog Number MAK194C
Hematoxylin 24 mL
Catalog Number MAK194D
membrane panel facing up and spin at 5,000 × g maximum for
approximately 15–60 minutes.
NOTE: Refer to Figures 1 and 2, and Tables 1 and 2 for typical spin times.
4. To recover the concentrated solute, insert
Boyer, ed.). Academic Press (New York
and London), pp. 562-608 (1971).
2. Jacobs, M. et al., Nucleic Acids Res., 13(24),
8913-8926 (1985).
3. Ottesen, M., and Svendsen, I., Methods Enzymol.
sequencing products):
1. 95 °C for 5 minutes
2. 95 °C for 30 seconds
3. 50 °C for 10 seconds
4. 60 °C for 4 minutes
5. Repeat steps 2–4 for a total of 100
assay, add 24 µL to 36 µL water).
Using seven glycerol – free tubes, perform 2-fold serial dilutions from 1:2.5 – 1:160, using
Incubation Solution + 2% BSA as the diluent. For example, add 60