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Waldemar Waldeck et al.
International journal of medical sciences, 8(2), 97-105 (2011-02-01)
Fluorescent proteins (FPs) are established tools for new applications, not-restricted to the cell biological research. They could also be ideal in surgery enhancing the precision to differentiate between the target tissue and the surrounding healthy tissue. FPs like the KillerRed
Maria Strianese et al.
Protein and peptide letters, 18(3), 282-286 (2010-09-23)
A new, fast, simple and cost-effective sensing device for monitoring H(2)S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H(2)S detection are
Julian Weichsel et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 77(1), 52-63 (2009-11-10)
The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown.
Annedore Punge et al.
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100
Sophie Roizard et al.
Journal of the American Chemical Society, 133(42), 16868-16874 (2011-09-14)
G-protein-coupled receptors (GPCRs) are ubiquitous mediators of signal transduction across cell membranes and constitute a very important class of therapeutic targets. In order to study the complex biochemical signaling network coupling to the intracellular side of GPCRs, it is necessary
Dominik Wildanger et al.
Optics express, 16(13), 9614-9621 (2008-06-26)
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate
Anna Kułakowska et al.
Journal of fluorescence, 20(2), 563-569 (2009-12-30)
In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching
Benjamin Strauss et al.
Nucleic acids research, 40(2), 861-870 (2011-09-16)
Chemical probing is a common method for the structural characterization of RNA. Typically, RNA is radioactively end-labelled, subjected to probing conditions, and the cleavage fragment pattern is analysed by gel electrophoresis. In recent years, many chemical modifications, like fluorophores, were
Ronny Schmidt et al.
Journal of proteome research, 10(3), 1316-1322 (2011-01-21)
Based on a single-molecule sensitive fluorescence-linked immunosorbent assay, an analytical platform for the detection of lipoarabinomannan (LAM), a lipopolysaccharide marker of tuberculosis, was established that is about 3 orders of magnitude more sensitive than comparable current ELISA assays. No amplification
Thomas D Lazzara et al.
Journal of colloid and interface science, 366(1), 57-63 (2011-10-29)
Anodic aluminum oxide (AAO) substrates with aligned, cylindrical, non-intersecting pores with diameters of 75 nm and depths of 3.5 or 10 μm were functionalized with lipid monolayers harboring different receptor lipids. AAO was first functionalized with dodecyl-trichlorosilane, followed by fusion
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