Showing 1-30 of 38 resultados for "A0157"
Alexey Potapov et al.
Physical chemistry chemical physics : PCCP, 12(1), 62-65 (2009-12-22)
Ascorbate oxidase contains two paramagnetic Cu(ii) binding sites, type 1 (T1) and type 2 (T2) and in both sites the Cu(ii) is coordinated to histidine residues. We use several pulse EPR techniques at high field (95 GHz) to determine ligand...
Jing Cui et al.
Archives of environmental contamination and toxicology, 59(1), 100-108 (2009-12-08)
Napropamide is a widely used herbicide for controlling weeds in crop production. However, extensive use of the herbicide has led to its accumulation in ecosystems, thus causing toxicity to crops and reducing crop production and quality. Salicylic acid (SA) plays...
Mara Fiorani et al.
The Journal of nutritional biochemistry, 24(2), 467-474 (2012-05-29)
Exposure of U937 cells to low micromolar levels of ascorbic acid or dehydroascorbic acid, while resulting in identical ascorbic acid accumulation, is unexpectedly associated with remarkably different responses to exogenous oxidants. We observed that otherwise nontoxic levels of hydrogen peroxide...
Emiko Kasahara et al.
The Biochemical journal, 421(2), 293-299 (2009-04-24)
Because AA (L-ascorbic acid) scavenges various types of free radicals to form MDAA (monodehydroascorbic acid) and DAA (dehydroascorbic acid), its regeneration from the oxidized metabolites is critically important for humans and other animals that lack the ability to synthesize this...
Cotton ascorbate oxidase promotes cell growth in cultured tobacco bright yellow-2 cells through generation of apoplast oxidation
Li R, et al.
International Journal of Molecular Sciences, 18(7), 1346-1346 (2017)
Mahmoud Orazizadeh et al.
Cell journal, 17(3), 412-421 (2015-10-16)
Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in sunscreens, bio- sensors, food additives, pigments, manufacture of rubber products, and electronic materi- als. There are several studies about the effects of NPs on dermal fibroblast or keratino- cytes, but very little...
Seung-Rock Lee et al.
Journal of microbiology (Seoul, Korea), 47(2), 178-186 (2009-05-05)
A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can...
Copper metalloenzymes
Messerschmidt A
Comprehensive Natural Products II: Chemistry and Biology (2010)
Shikha Pundir et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 39(5), 324-329 (2011-04-22)
Abstract: An ascorbate oxidase purified from the green fruit of zucchini squash (Cucurbita pepo medullosa) was immobilized photochemically on a polyethylene disc with 85% retention of initial activity of free enzyme. The optimum pH (5.5) was unchanged, while K(m) was...
Ming Liu et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 27(5), 477-477 (2011-05-12)
An amperometric L-ascorbic acid (AA) biosensor fabricated by immobilizing ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT) and multi-walled carbon nanotubes (MWCNTs) composite films was reported for the first time. The entrapment of AO in PEDOT/MWCNTs composite films was performed during an...
Attila Vig et al.
Bioprocess and biosystems engineering, 33(8), 947-952 (2010-03-13)
An amperometric detector and an enzymatic reaction were combined for the measurement of L-ascorbic acid. The enzyme cell (containing immobilized ascorbate oxidase) was connected to a flow injection analyzer (FIA) system with a glassy carbon electrode as an amperometric detector....
Manuel A Matamoros et al.
Journal of experimental botany, 61(1), 87-97 (2009-10-14)
In plant cells, antioxidants keep reactive oxygen species at low concentrations, avoiding oxidative damage while allowing them to play crucial functions in signal transduction. However, little is known about the role of antioxidants during fruit maturation, especially in legumes. Snap...
Xiao-Yan Yang et al.
Journal of plant physiology, 168(18), 2196-2205 (2011-09-20)
Citrus fruit is widely consumed and provides ascorbate for human health. The ascorbate content in pulp is generally higher in orange (Citrus sinensis Osb.) than in Satsuma mandarin (Citrus unshiu Marc.). However, what contributes to such difference is still unknown....
C S Pundir et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 39(3), 177-184 (2010-11-12)
Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin "Araldite" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to...
Zhenzhen Chen et al.
Analytical chemistry, 84(11), 4687-4694 (2012-05-04)
Determination of intracellular bioactive species will afford beneficial information related to cell metabolism, signal transduction, cell function, and disease treatment. In this study, the first application of a microchip electrophoresis-laser-induced fluorescence (MCE-LIF) method for concurrent determination of reactive oxygen species...
In vitro toxic effects of zinc oxide nanoparticles on rat adipose tissue-derived mesenchymal stem cells
Orazizadeh M, et al.
Cell Journal, 17(3), 412-412 (2015)
Ashish K Srivastava et al.
Plant physiology and biochemistry : PPB, 49(6), 676-686 (2011-03-23)
Thiourea (TU) has been found to enhance the stress tolerance of plants in our earlier field trials. In the present study, the TU mediated effect on the redox and antioxidant responses were studied in response to salinity (NaCl) stress in...
Virginie Garcia et al.
Comptes rendus biologies, 332(11), 1007-1021 (2009-11-17)
Very few reports have studied the interactions between ascorbate and fruit metabolism. In order to get insights into the complex relationships between ascorbate biosynthesis/recycling and other metabolic pathways in the fruit, we undertook a fruit systems biology approach. To this...
Shuichi Hara et al.
Toxicology, 264(1-2), 69-73 (2009-07-28)
Carbon monoxide (CO) poisoning stimulated generation in rat striatum of toxic hydroxyl radicals (*OH), which might participate in the CO-induced neuronal injury. Since an increase in extracellular ascorbate (AA) stimulated *OH generation in the presence of endogenous metals, including iron...
Mechanism of free radical formation and disappearance during the ascorbic acid oxidase and peroxidase reactions.
Biochimica et biophysica acta, 50, 62-69 (1961-06-10)
Cécile Garchery et al.
Plant, cell & environment, 36(1), 159-175 (2012-06-26)
The regulation of carbon allocation between photosynthetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool. RNA...
A Messerschmidt et al.
Journal of molecular biology, 224(1), 179-205 (1992-03-05)
The crystal structure of the fully oxidized form of ascorbate oxidase (EC from Zucchini has been refined at 1.90 A (1 A = 0.1 nm) resolution, using an energy-restrained least-squares refinement procedure. The refined model, which includes 8764 protein...
Irina I Panchuk et al.
Plant physiology, 129(2), 838-853 (2002-06-18)
To find evidence for a connection between heat stress response, oxidative stress, and common stress tolerance, we studied the effects of elevated growth temperatures and heat stress on the activity and expression of ascorbate peroxidase (APX). We compared wild-type Arabidopsis...
Yoshiaki Ueda et al.
Plant physiology and biochemistry : PPB, 70, 418-423 (2013-07-10)
Ascorbate (AsA) is an important metabolite involved in stress response and development of plants. Therefore it is necessary to quantify the AsA content in many fields of plant science, including high throughput and critical applications. In this study we compared...
Fiona L Goggin et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 32(9), 777-790 (2010-07-29)
Analysis of a diverse cross-sample of plant-insect interactions suggests that the abundance of vitamin C (L-ascorbic acid, ascorbate or AsA) in plants influences their susceptibility to insect feeding. These effects may be mediated by AsAs roles as an essential dietary...
Kensaku Suzuki et al.
Plant, cell & environment, 38(7), 1255-1274 (2014-12-17)
In rice (Oryza sativa L.), chilling-induced male sterility increased when plants experienced low water temperature (Tw , 18 °C for 14 d) before panicle initiation. The number of mature pollen grains after chilling at the booting stage (12 °C for...
Sudhakar Srivastava et al.
Protoplasma, 248(4), 805-815 (2010-12-29)
Arsenic (As) is a potential hazard to plants' health, however the mechanisms of its toxicity are yet to be properly understood. To determine the impact of redox state and energetic in stress imposition, plants of Hydrilla verticillata (L.f.) Royle, which...
Direct electrochemistry of proteins and enzymes
Perspectives in bioanalysis, 1, 517-598 (2005)
Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Sewelam N, et al.
PLoS ONE, 8(8), e70289-e70289 (2013)
T S Porto et al.
Applied biochemistry and biotechnology, 160(4), 1057-1064 (2009-05-05)
The ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. The techniques currently used for the purification of this enzyme raise its production cost. Thus, the development...

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