Showing 1-30 of 411 resultados for "

a2095

"
Prashant Monian et al.
The Journal of biological chemistry, 291(5), 2379-2388 (2015-12-17)
A signature event during the cell intrinsic apoptotic pathway is mitochondrial outer membrane permeabilization, leading to formation of the apoptosome, a caspase activation complex. The cellular apoptosis susceptibility protein (CAS) can facilitate apoptosome assembly by stimulating nucleotide exchange on Apaf-1...
Sandra Goetze et al.
PLoS biology, 7(11), e1000236-e1000236 (2009-11-04)
Protein modifications play a major role for most biological processes in living organisms. Amino-terminal acetylation of proteins is a common modification found throughout the tree of life: the N-terminus of a nascent polypeptide chain becomes co-translationally acetylated, often after the...
Matt Greenlee et al.
Cytoskeleton (Hoboken, N.J.), 75(7), 290-306 (2018-05-08)
Stu2p is the yeast member of the XMAP215/Dis1/ch-TOG family of microtubule-associated proteins that promote microtubule polymerization. However, the factors that regulate its activity are not clearly understood. Here we report that Stu2p in the budding yeast Saccharomyces cerevisiae interacts with...
Tao Yun et al.
The Journal of biological chemistry, 291(14), 7386-7395 (2016-02-07)
The p53 tumor suppressor controls cell growth, metabolism, and death by regulating the transcription of various target genes. The target-specific transcriptional activity of p53 is highly regulated. Here we demonstrate that acetylation of p53 at Lys-120 up-regulates its transcriptional activity...
Tomoaki Ohtsuka et al.
Scientific reports, 6, 39557-39557 (2016-12-23)
HER2 is a receptor tyrosine kinase and its upregulation via activating mutations or amplification has been identified in some malignant tumors, including lung cancers. Because HER2 can be a therapeutic target in HER2-driven malignancies, it is important to understand the...
null
Hiroshi Qadota et al.
The Journal of biological chemistry, 287(34), 28537-28551 (2012-07-05)
Caenorhabditis elegans muscle cells attach to basement membrane through adhesion plaques. PAT-3 (β-integrin), UNC-112 (kindlin), and PAT-4 (integrin-linked kinase) are associated with these structures. Genetic analysis indicated that PAT-4 is required for UNC-112 to be properly localized. We investigated the...
Jackson L K Yip et al.
Molecules (Basel, Switzerland), 25(21) (2020-11-12)
Adult neurogenesis is modulated by many Gi-coupled receptors but the precise mechanism remains elusive. A key step for maintaining the population of neural stem cells in the adult is asymmetric cell division (ACD), a process which entails the formation of...
Elizabeth A White et al.
Journal of virology, 86(24), 13174-13186 (2012-09-28)
We have begun to define the human papillomavirus (HPV)-associated proteome for a subset of the more than 120 HPV types that have been identified to date. Our approach uses a mass spectrometry-based platform for the systematic identification of interactions between...
Laura Vuolo et al.
eLife, 7 (2018-10-16)
The dynein-2 microtubule motor is the retrograde motor for intraflagellar transport. Mutations in dynein-2 components cause skeletal ciliopathies, notably Jeune syndrome. Dynein-2 contains a heterodimer of two non-identical intermediate chains, WDR34 and WDR60. Here, we use knockout cell lines to...
Xiaowen Wang et al.
Nature, 524(7566), 481-484 (2015-07-21)
Mitochondria are multifunctional organelles whose dysfunction leads to neuromuscular degeneration and ageing. The multi-functionality poses a great challenge for understanding the mechanisms by which mitochondrial dysfunction causes specific pathologies. Among the leading mitochondrial mediators of cell death are energy depletion...
Zhenping Cao et al.
Nature microbiology, 2, 16183-16183 (2016-10-11)
The type VII protein secretion system (T7SS) plays a critical role in the virulence of human pathogens including Mycobacterium tuberculosis and Staphylococcus aureus. Here, we report that the S. aureus T7SS secretes a large nuclease toxin, EsaD. The toxic activity...
Hung Thanh Nguyen et al.
Cell discovery, 2, 16001-16001 (2016-07-28)
The Hippo pathway has been identified as a key barrier for tumorigenesis, acting through downregulation of YAP/TAZ activity. Elevated YAP/TAZ activity has been documented in many human cancers. Ubiquitylation has been shown to play a key role in regulating YAP/TAZ...
Shiv Gandhi et al.
Cell chemical biology, 27(11), 1410-1424 (2020-09-06)
Rhomboid intramembrane proteases regulate pathophysiological processes, but their targeting in a disease context has never been achieved. We decoded the atypical substrate specificity of malaria rhomboid PfROM4, but found, unexpectedly, that it results from "steric exclusion": PfROM4 and canonical rhomboid...
Ritu Garg et al.
Journal of cell science, 133(6) (2020-02-12)
Rnd3 is an atypical Rho family protein that is constitutively GTP bound, and acts on membranes to induce loss of actin stress fibers and cell rounding. Phosphorylation of Rnd3 promotes 14-3-3 binding and its relocation to the cytosol. Here, we...
Lu Liu et al.
Cell reports, 23(6), 1879-1890 (2018-05-10)
Organogenesis in higher plants occurs in the shoot meristem, which contains pluripotent stem cells. Here, we show that two multiple C2 domain and transmembrane region proteins, FT INTERACTING PROTEIN 3 (FTIP3) and FTIP4, play an essential role in mediating proliferation...
null
Eri Miyagi et al.
Journal of virology, 88(9), 4839-4852 (2014-02-14)
Vif is a lentiviral accessory protein that regulates viral infectivity in part by inducing proteasomal degradation of APOBEC3G (A3G). Recently, CBFβ was found to facilitate Vif-dependent degradation of A3G. However, the exact role of CBFβ remains unclear. Several studies noted...
Bo Yang et al.
Viruses, 12(2) (2020-02-28)
Infectious bursal disease (IBD) is an immunosuppressive, highly contagious, and lethal disease of young chickens caused by IBD virus (IBDV). It results in huge economic loss to the poultry industry worldwide. Infection caused by very virulent IBDV (vvIBDV) strains results...
Kristy J Wilson et al.
Molecular biology of the cell, 23(14), 2623-2634 (2012-05-25)
The ubiquitin proteasome system is involved in degradation of old or damaged sarcomeric proteins. Most E3 ubiquitin ligases are associated with cullins, which function as scaffolds for assembly of the protein degradation machinery. Cullin 3 uses an adaptor to link...
Soham Gupta et al.
PLoS pathogens, 15(11), e1008146-e1008146 (2019-11-12)
The 14-3-3 molecular scaffolds promote type I interferon (IFN) responses by stabilizing the interaction of RIG-I with the TRIM25 ligase. Viruses have evolved unique strategies to halt this cellular response to support their replication and spread. Here, we report that...
Michael A Davis et al.
Genes & development, 27(2), 151-156 (2013-01-17)
The Mediator complex is an essential transcription regulator that bridges transcription factors with RNA polymerase II. This interaction is controlled by dynamic interactions between Mediator and the CDK8 module, but the mechanisms governing CDK8 module-Mediator association remain poorly understood. We...
Min Mo et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(46), 19527-19532 (2009-11-06)
The anaphase-promoting complex (APC), or cyclosome, is a ubiquitin ligase with major roles in cell cycle regulation. It is required for mitotic exit, but must be deactivated for the G(1)/S phase transition to occur. APC consists of at least 12...
Nan Su et al.
The Journal of biological chemistry, 283(50), 35106-35117 (2008-10-23)
C/EBP homology protein (CHOP), a stress-induced transcription factor, is involved in transcriptional regulation, cell cycle, and apoptosis. The present studies identified CHOP as an interacting partner of activating transcription factor (ATF) 4 in a yeast two-hybrid screen and confirmed their...
Nikita M Shepelev et al.
Scientific reports, 10(1), 11109-11109 (2020-07-08)
Telomerase is a ribonucleoprotein enzyme, which maintains genome integrity in eukaryotes and ensures continuous cellular proliferation. Telomerase holoenzyme from the thermotolerant yeast Hansenula polymorpha, in addition to the catalytic subunit (TERT) and telomerase RNA (TER), contains accessory proteins Est1 and...
Andriana Margariti et al.
Circulation research, 106(7), 1202-1211 (2010-03-13)
Histone deacetylase (HDAC)7 is expressed in the early stages of embryonic development and may play a role in endothelial function. This study aimed to investigate the role of HDAC7 in endothelial cell (EC) proliferation and growth and the underlying mechanism....
Jianping Guo et al.
Nature cell biology, 21(2), 226-237 (2019-01-30)
Aberrant activation of AKT disturbs the proliferation, survival and metabolic homeostasis of various human cancers. Thus, it is critical to understand the upstream signalling pathways governing AKT activation. Here, we report that AKT undergoes SETDB1-mediated lysine methylation to promote its...
Irene Santos-Barriopedro et al.
Genes & cancer, 10(5-6), 119-133 (2019-12-05)
Temozolomide (TMZ) is an alkylating agent chemotherapy drug used as a first-line treatment for glioblastoma multiforme (GBM). O6-methyl-guanine DNA methyltransferase (MGMT) repairs DNA damage induced by TMZ; hence, elevated MGMT levels usually correlate with TMZ resistance. MGMT promoter methylation is...
null
Prashant K Mishra et al.
Cell cycle (Georgetown, Tex.), 17(1), 11-23 (2017-10-06)
Shugoshin is an evolutionarily conserved protein, which is involved in tension sensing on mitotic chromosomes, kinetochore biorientation, and protection of centromeric (CEN) cohesin for faithful chromosome segregation. Interaction of the C-terminus of Sgo1 with phosphorylated histone H2A regulates its association...