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Juan Antonio Tamayo-Ramos et al.
Biotechnology and bioengineering, 110(2), 543-551 (2012-09-06)
In filamentous fungi, most of the strategies used for the improvement of protein yields have been based on an increase in the transcript levels of a target gene. Strategies focusing at the translational level have been also described, but are
Swati B Jadhav et al.
Carbohydrate polymers, 98(1), 1191-1197 (2013-08-31)
Two enzymes, α-amylase and glucoamylase have been individually and co-conjugated to pectin by covalent binding. Both the enzyme systems showed better thermal and pH stability over the free enzyme system with the complete retention of original activities. Mixture of individually
The maltase of Clostridium acetobutylicum; its specificity range and mode of action.
D FRENCH et al.
The Journal of biological chemistry, 187(2), 463-471 (1950-12-01)
Han-bing Zhang et al.
Zhonghua er ke za zhi = Chinese journal of pediatrics, 50(6), 415-419 (2012-08-31)
To analyze and summarize the characteristics of glycogen storage disease type II (Pompe disease) patients according to the clinical description and prognosis. Seventeen Chinese patients diagnosed by acid alpha-glucosidase (GAA) enzyme activity test were reviewed. Clinical data tables were designed.
The subcellular distribution of enzymes in type II glycogenosis and the occurrence of an oligo-alpha-1,4-glucan glucohydrolase in human tissues.
B I Brown et al.
Biochimica et biophysica acta, 110(1), 124-133 (1965-10-25)
Pierre-Emmanuel Tardo-Dino et al.
Physiological reports, 9(16), e14686-e14686 (2021-08-19)
Aerobic training leads to well-known systemic metabolic and muscular alterations. Heat acclimation may also increase mitochondrial muscle mass. We studied the effects of heat acclimation combined with endurance training on metabolic adaptations of skeletal muscle. Thirty-two rats were divided into
Shay-Whey M Koh et al.
Journal of neurochemistry, 109(3), 792-806 (2009-03-03)
The neuropeptide vasoactive intestinal peptide (VIP) is anti-inflammatory and protective in the immune and nervous systems, respectively. This study demonstrated in corneal endothelial (CE) cells injured by severe oxidative stress (1.4 mM H(2)O(2)) in bovine corneal organ cultures that VIP
Thomas Hundsberger et al.
Journal of neurology, 260(9), 2279-2285 (2013-06-12)
Glycogen storage disease type II is a rare multi-systemic disorder characterised by an intracellular accumulation of glycogen due a mutation in the acid alpha glucosidase (GAA) gene. The level of residual enzyme activity, the genotype and other yet unknown factors
Yi-Chen Lin et al.
PloS one, 12(5), e0177115-e0177115 (2017-05-05)
It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific
Youngmi Kim et al.
Bioresource technology, 99(12), 5165-5176 (2007-11-09)
DDGS and wet distillers' grains are the major co-products of the dry grind ethanol facilities. As they are mainly used as animal feed, a typical compositional analysis of the DDGS and wet distillers' grains mainly focuses on defining the feedstock's
Sachin Talekar et al.
Bioresource technology, 147, 269-275 (2013-09-04)
A tri-enzyme biocatalyst "combi-CLEAs" with starch hydrolytic activity was prepared from commercially available alpha amylase, glucoamylase and pullulanase preparations by aggregating enzymes with ammonium sulphate followed by cross-linking formed aggregates for 4.5h with 40 mM glutaraldehyde. The effects of precipitant
A blood trans-alpha-glucosylase.
Biochimica et biophysica acta, 22(1), 193-194 (1956-10-01)
Adam J Dobson et al.
Nature communications, 6, 6312-6312 (2015-02-19)
Animals bear communities of gut microorganisms with substantial effects on animal nutrition, but the host genetic basis of these effects is unknown. Here we use Drosophila to demonstrate substantial among-genotype variation in the effects of eliminating the gut microbiota on
María Clara Ingaramo et al.
Cell reports, 33(4), 108321-108321 (2020-10-29)
The tumor suppressor p53 regulates multiple metabolic pathways at the cellular level. However, its role in the context of a whole animal response to metabolic stress is poorly understood. Using Drosophila, we show that AMP-activated protein kinase (AMPK)-dependent Dmp53 activation
Carolina Bravo et al.
International journal of food sciences and nutrition, 71(5), 604-613 (2019-11-21)
Glycaemic response (GR) to starch-based meals depends on their food composition and microstructure. We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility. Additionally, a pilot
Enrico Bertaggia et al.
American journal of physiology. Endocrinology and metabolism, 313(2), E121-E133 (2017-04-06)
Bile acids (BAs) are cholesterol derivatives that regulate lipid metabolism, through their dual abilities to promote lipid absorption and activate BA receptors. However, different BA species have varying abilities to perform these functions. Eliminating 12α-hydroxy BAs in mice via
Tiziana Ventriglia et al.
Plant physiology, 148(1), 65-76 (2008-07-11)
ADP-glucose (Glc) pyrophosphorylase (ADP-Glc PPase) catalyzes the first committed step in starch biosynthesis. Higher plant ADP-Glc PPase is a heterotetramer (alpha(2)beta(2)) consisting of two small and two large subunits. There is increasing evidence that suggests that catalytic and regulatory properties
Long Chen et al.
Food chemistry, 242, 131-138 (2017-10-19)
Concerns regarding increased dietary oil uptake have prompted efforts to investigate the oil absorption and distribution in fried starchy foods. In the present study, attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, together with a chloroform-methanol method, was used to
Alisa K Manning et al.
Frontiers in genetics, 11, 615-615 (2020-08-06)
Causal transcripts at genomic loci associated with type 2 diabetes (T2D) are mostly unknown. The chr8p23.1 variant rs4841132, associated with an insulin-resistant diabetes risk phenotype, lies in the second exon of a long non-coding RNA (lncRNA) gene, LOC157273, located 175
Ting-Ying Jiang et al.
PloS one, 7(7), e41131-e41131 (2012-07-21)
The N-terminal starch binding domain of Rhizopus oryzae glucoamylase (RoSBD) has a high binding affinity for raw starch. RoSBD has two ligand-binding sites, each containing a ligand-binding clamp: a polyN clamp residing near binding site I is unique in that
Joto Yoshimoto et al.
Nutrients, 12(6) (2020-06-25)
Legumes are low-carbohydrate food and are abundant in dietary fiber. In order to provide a functional staple food that does not cause a rapid increase in postprandial blood glucose levels, four kinds of legumes were focused on as ingredients. Noodles
P M Nunes et al.
American journal of physiology. Endocrinology and metabolism, 303(1), E71-E81 (2012-04-13)
Adipose triglyceride lipase (ATGL) is a lipolytic enzyme that is highly specific for triglyceride hydrolysis. The ATGL-knockout mouse (ATGL(-/-)) accumulates lipid droplets in various tissues, including skeletal muscle, and has poor maximal running velocity and endurance capacity. In this study
Zaki A El-Fiky et al.
Journal of food science, 77(6), M312-M317 (2012-05-16)
Baker's yeast, Saccharomyces cerevisiae, is a key component in bread baking. Total of 12 commercial baker's yeast and 2 hybrid strains were compared using traditional quality parameters. Total of 5 strains with high leavening power and the 2 hybrid strains
Chao Zhang et al.
Ecotoxicology (London, England), 29(2), 175-184 (2020-01-16)
A key challenge for ecological risk assessment of contaminants under global warming is to predict effects at higher levels of biological organisation. One approach to reach this goal is to study how contaminants and warming cause changes in body stoichiometry
Egberto Gaspar de Moura et al.
The Journal of physiology, 587(Pt 20), 4919-4929 (2009-09-09)
Neonatal malnutrition is associated with metabolic syndrome in adulthood. Maternal hypoprolactinaemia at the end of lactation (a precocious weaning model) caused obesity, leptin resistance and hypothyroidism in adult offspring, suggesting an association of prolactin (PRL) and programming of metabolic dysfunctions.
Lizanne Janssens et al.
Aquatic toxicology (Amsterdam, Netherlands), 193, 210-216 (2017-11-04)
Pesticides are causing strong decreases in aquatic biodiversity at concentrations assumed safe by legislation. One reason for the failing risk assessment may be strong differences in the toxicity of the active ingredient of pesticides and their commercial formulations. Sublethal effects
Shizhe Tang et al.
Journal of the science of food and agriculture, 100(7), 3013-3023 (2020-02-15)
Mesophilic α-amylases function effectively at low temperatures with high rates of catalysis and require less energy for starch hydrolysis. Bacillus amyloliquefaciens is an essential producer of mesophilic α-amylases. However, because of the existence of the restriction-modification system, introducing exogenous DNAs
Kun Lyu et al.
Cell metabolism, 32(4), 654-664 (2020-09-04)
Nonalcoholic fatty liver disease is strongly associated with hepatic insulin resistance (HIR); however, the key lipid species and molecular mechanisms linking these conditions are widely debated. We developed a subcellular fractionation method to quantify diacylglycerol (DAG) stereoisomers and ceramides in
Thiago R Araujo et al.
Journal of cellular physiology, 234(5), 7019-7031 (2018-10-15)
Obesity predisposes to glucose intolerance and type 2 diabetes (T2D). This disease is often characterized by insulin resistance, changes in insulin clearance, and β-cell dysfunction. However, studies indicate that, for T2D development, disruptions in glucagon physiology also occur. Herein, we
Trine Lisberg Toft-Bertelsen et al.
Glia, 69(1), 28-41 (2020-06-09)
The mammalian brain consists of 80% water, which is continuously shifted between different compartments and cellular structures by mechanisms that are, to a large extent, unresolved. Aquaporin 4 (AQP4) is abundantly expressed in glia and ependymal cells of the mammalian
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