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Palabra clave:'c4706'
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c4706

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Remi Hatinguais et al.
Frontiers in immunology, 12, 641495-641495 (2021-04-13)
Reactive Oxygen Species (ROS) are highly reactive molecules that can induce oxidative stress. For instance, the oxidative burst of immune cells is well known for its ability to inhibit the growth of invading pathogens. However, ROS also mediate redox signalling
Lingzhi Liu et al.
Biosensors & bioelectronics, 70, 351-357 (2015-04-05)
Measurement of microRNA (miRNA) levels in body fluids is a crucial tool for the early diagnosis and prognosis of cancers. In this study, we developed an electrochemical assay to detect miRNA-21 by fabricating the electrode with layer-by-layer assembly of oxidized
Cynthia M Chio et al.
Chembiochem : a European journal of chemical biology, 16(12), 1735-1739 (2015-06-13)
Few chemical strategies for activating enzymes have been developed. Here we show that a biarsenical compound (FlAsH) can directly activate a rationally engineered protein tyrosine phosphatase (Shp2 PTP) by disrupting autoinhibitory interactions between Shp2's N-terminal SH2 domain and its PTP
Miguel Angel García-Sevillano et al.
Analytical and bioanalytical chemistry, 406(24), 5853-5865 (2014-07-30)
The biochemical response of mice (Mus musculus) to acute subcutaneous inorganic-mercury exposure was assessed over a 14-day period by analyzing cytosolic extracts of the liver, the kidneys, and blood plasma. Integrated metallomic and metabolomic approaches using elemental and molecular-mass spectrometry
Santosh Renuse et al.
EBioMedicine, 69, 103465-103465 (2021-07-07)
The COVID-19 pandemic caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has overwhelmed health systems worldwide and highlighted limitations of diagnostic testing. Several types of diagnostic tests including RT-PCR-based assays and antigen detection by lateral flow assays, each with their
Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure
Maria Chiara Barsotti et al.
Journal of biomedical materials research. Part A, 103(10), 3284-3292 (2015-03-27)
As the endothelium still represents the ideal surface for cardiovascular devices, different endothelialization strategies have been attempted for biocompatibility and nonthrombogenicity enhancement. Since endothelial progenitor cells (EPCs) could accelerate endothelialization, preventing thrombosis and restenosis, the aim of this study was
Guohong Gan et al.
Molecular & cellular proteomics : MCP, 20, 100051-100051 (2021-02-08)
SDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC-MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have
H Robert Bergen et al.
Skeletal muscle, 5, 21-21 (2015-07-17)
Myostatin is a protein synthesized and secreted by skeletal muscle that negatively regulates muscle mass. The extent to which circulating myostatin levels change in the context of aging is controversial, largely due to methodological barriers. We developed a specific and
Qi Li et al.
Cell stem cell, 26(5), 675-692 (2020-04-08)
Intestinal homeostasis is tightly regulated by complex yet poorly understood signaling networks. Here, we demonstrate that Lats1/2, the core Hippo kinases, are essential to maintain Wnt pathway activity and intestinal stem cells. Lats1/2 deletion leads to loss of intestinal stem
Andrew M Leidal et al.
Nature cell biology, 22(2), 187-199 (2020-01-15)
Traditionally viewed as an autodigestive pathway, autophagy also facilitates cellular secretion; however, the mechanisms underlying these processes remain unclear. Here, we demonstrate that components of the autophagy machinery specify secretion within extracellular vesicles (EVs). Using a proximity-dependent biotinylation proteomics strategy
Huiyan Li et al.
Talanta, 146, 727-731 (2015-12-24)
We present an aptamer-based microcantilever biosensor for label-free detection of nucleolin. The sensor cantilevers in the microcantilever array were functionalized with nucleolin aptamer (AS1411) while the reference cantilevers were modified by 6-mercapto-1-hexanol (MCH) to eliminate environmental disturbances. The interaction between
Zhanpeng Zhang et al.
Advanced functional materials, 25(3), 350-360 (2015-06-13)
Injectable microspheres are attractive stem cell carriers for minimally invasive procedures. For tissue regeneration, the microspheres need to present the critical cues to properly direct stem cell differentiation. In natural extracellular matrix (ECM), growth factors (GFs) and collagen nanofibers provide
Hansjörg Götzke et al.
Nature communications, 10(1), 4403-4403 (2019-09-29)
Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed epitope tag that serves a remarkably broad spectrum of applications in life sciences while
Keli Chai et al.
Viruses, 13(1) (2020-12-31)
Membrane proteins of enveloped viruses have been reported to undergo palmitoylation, a post-translational modification often having a critical role in the function of these viral proteins and hence viral replication. In this study, we report that the foamy virus (FV)
Nicholas A Swanson et al.
STAR protocols, 2(4), 100960-100960 (2021-11-27)
Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery.
Ravi R Sonani et al.
Photosynthesis research, 144(3), 349-360 (2020-04-19)
The crystal structure of phycocyanin (pr-PC) isolated from Phormidium rubidum A09DM (P. rubidum) is described at a resolution of 1.17 Å. Electron density maps derived from crystallographic data showed many clear differences in amino acid sequences when compared with the previously
Yan Li et al.
Biosensors & bioelectronics, 67, 364-369 (2014-09-10)
The development of sensitive telomerase biosensors is hindered by the restricted accessibility of telomere strand (TS) primer and the limited enzyme reaction space, which is mainly confined by the vertical distance. In this work, we designed an electrochemical telomerase biosensor
Patanachai Limpikirati et al.
mAbs, 11(3), 463-476 (2019-01-15)
Monoclonal antibodies are among the fastest growing therapeutics in the pharmaceutical industry. Detecting higher-order structure changes of antibodies upon storage or mishandling, however, is a challenging problem. In this study, we describe the use of diethylpyrocarbonate (DEPC)-based covalent labeling (CL)
Patanachai K Limpikirati et al.
Journal of the American Society for Mass Spectrometry, 31(6), 1223-1232 (2020-04-21)
Diethylpyrocarbonate (DEPC)-based covalent labeling together with mass spectrometry is a promising tool for the higher-order structural analysis of antibody therapeutics. Reliable information about antibody higher-order structure can be obtained, though, only when the protein's structural integrity is preserved during labeling.
Husam Aljwaid et al.
Journal of diabetes and its complications, 29(7), 943-949 (2015-06-25)
To investigate the association between circulating non-transferrin-bound iron [NTBI], and markers of oxidative stress, endothelial function and inflammation in subjects with type 2 diabetes and non-diabetic subjects with varying degrees of obesity. Plasma NTBI was measured by HPLC, together with
Ying Liu et al.
Biosensors & bioelectronics, 64, 43-50 (2014-09-06)
Inflammatory cytokines are secreted by immune cells in response to infection or injury. Quantification of multiple cytokines in parallel may help with disease diagnosis by illuminating inflammatory pathways related to disease onset and progression. This paper describes development of an
Karl T Hansson et al.
Proteomics, 17(5) (2017-01-04)
Biomarkers of neurodegenerative disorders are needed to assist in diagnosis, to monitor disease progression and therapeutic interventions, and to provide insight into disease mechanisms. One route to identify such biomarkers is by proteomic and peptidomic analysis of cerebrospinal fluid (CSF).
Barbora Salovska et al.
Molecular systems biology, 16(3), e9170-e9170 (2020-03-17)
Profiling of biological relationships between different molecular layers dissects regulatory mechanisms that ultimately determine cellular function. To thoroughly assess the role of protein post-translational turnover, we devised a strategy combining pulse stable isotope-labeled amino acids in cells (pSILAC), data-independent acquisition
Sayed Habib Kazemi et al.
Materials science & engineering. C, Materials for biological applications, 52, 151-154 (2015-05-09)
In this article we report the application of non-Faradaic mode of electrochemical impedance spectroscopy (EIS) for determination of potassium ion (K(+)) concentration using a guanine rich K(+)-selective aptasensor (K(+)-aptasensor). This is a simple, electroactive probe free, sensitive and reproducible method
Hui Chan Kwak et al.
Biochemical pharmacology, 96(3), 256-266 (2015-06-07)
The present study was aimed to investigate the metabolomics of sulfur amino acids in Zucker diabetic fatty (ZDF) rats, an obese type 2 diabetic animal model. Plasma levels of total cysteine, homocysteine and methionine, but not glutathione (GSH) were markedly
Yvonne S Ziegler et al.
PloS one, 9(7), e102341-e102341 (2014-07-17)
The use of broad spectrum chemotherapeutic agents to treat breast cancer results in substantial and debilitating side effects, necessitating the development of targeted therapies to limit tumor proliferation and prevent metastasis. In recent years, the list of approved targeted therapies
Young-Chan Kim et al.
Nature chemical biology, 15(9), 907-916 (2019-08-21)
Toll-like receptor (TLR)/myeloid differentiation primary response protein (MYD88) signaling aggravates sepsis by impairing neutrophil migration to infection sites. However, the role of intracellular fatty acids in TLR/MYD88 signaling is unclear. Here, inhibition of fatty acid synthase by C75 improved neutrophil
Tris (2-carboxyethyl) phosphine Hydrochloride
Yost Julianne M et al.
Encyclopedia of Reagents for Organic Synthesis, Second Edition, 1-9 (2001)
Sean Chen et al.
Cell reports, 27(13), 3780-3789 (2019-06-27)
Genetically engineered mouse models harboring large sequence insertions or modifications are critical for a wide range of applications including endogenous gene tagging, conditional knockout, site-specific transgene insertion, and gene replacement; however, existing methods to generate such animals remain laborious and
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