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Protein and nucleic acid interactions

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Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
Using Duolink™ in Multiwell Plates
Duolink protocol modifications for staining cells in multiwell plates; adapt washing, imaging, and scanning for multiwell format.
Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol
RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.
Chromatin Isolation by RNA Purification (ChIRP) Protocol
Chromatin Isolation by RNA purification (ChIRP) protocol for isolating RNA-bound genome regions.
Creating PLA® Probes
Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.
Synthetic Peptide Handling & Storage Protocol
Solvent choice is crucial for synthetic peptide handling, with guidelines provided for proper dissolution.
Duolink® PLA Brightfield Protocol
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.