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Showing 1-11 of 11 results
Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber
Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt
Workflow for High-Throughput Glycoprofiling of Rituximab
Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Workflow for Antibody Aggregate and Fragment Analysis
A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.
Mass Spectrometry of Glycans
The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies.
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Production of 15N-13C Dual-labelled Proteins
Dr. Alan Robertson and Dr. Ben Davis at Vernalis, Ltd., UK, a provider of drug discovery and development services, have refined a novel protocol for the duallabelling of proteins using EnPresso® B Defined Nitrogen-free.
LC/MS/MS Analysis of Aflatoxins in Cannabis on Ascentis® Express 2.7µm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE
solution, 2 μg/mL in acetonitrile, analytical standard
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters
Biomarkers play an essential role in the drug discovery and development process.