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p0290

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Anna Wolters et al.
Neural regeneration research, 16(11), 2243-2249 (2021-04-06)
One of the major challenges in emergency medicine is out-of-hospital cardiac arrest (OHCA). Every year, about 53-62/100 000 people worldwide suffer an out-of-hospital cardiac arrest with serious consequences, whereas persistent brain injury is a major cause of morbidity and mortality
Natacha Merindol et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 128, 104423-104423 (2020-05-18)
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR
Zhen-Yu Li et al.
Journal of ethnopharmacology, 140(1), 83-90 (2012-01-03)
Flower bud of Tussilago farfara L. is widely used for the treatment of cough, bronchitis and asthmatic disorders in the Traditional Chinese Medicine. However, due to the increasing demands, adulteration with rachis is frequently encountered in the marketplace. No report
Vanicha Vichai et al.
Nature protocols, 1(3), 1112-1116 (2007-04-05)
The sulforhodamine B (SRB) assay is used for cell density determination, based on the measurement of cellular protein content. The method described here has been optimized for the toxicity screening of compounds to adherent cells in a 96-well format. After
Lengxob Yong et al.
The Journal of experimental biology, 220(Pt 17), 3017-3021 (2017-06-18)
Elucidating the genes that contribute to behavioral variation has become an important endeavor in behavioral studies. While advances in genomics have narrowed down the list of candidate genes, functional validation of them has lagged behind, partly because of challenges associated
Olaia F Vila et al.
Theranostics, 9(5), 1232-1246 (2019-03-15)
The study of human neuromuscular diseases has traditionally been performed in animal models, due to the difficulty of performing studies in human subjects. Despite the unquestioned value of animal models, inter-species differences hamper the translation of these findings to clinical
Ngoc Tung Tran et al.
STAR protocols, 1(1), 100028-100028 (2020-07-21)
Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse
Héctor Rubén Hernández-Garcés et al.
Revista espanola de enfermedades digestivas : organo oficial de la Sociedad Espanola de Patologia Digestiva, 104(1), 4-9 (2012-02-04)
An analytic study to validate a diagnostic test was carried out at the Institute of Gastroenterology in Havana, Cuba in adult patients of both sexes in whom chromoendoscopy was carried out with red phenol at 0.1% over the gastric mucosa
Parviz Lalezari et al.
Analytical biochemistry, 416(1), 92-99 (2011-05-25)
A new method for extraction and concentration of organic dyes that uses a reagent composed of a nonionic detergent mixed with an alcohol is described. We have observed that water-soluble organic dyes are also soluble in nonionic detergents and can
Paweł Kordowitzki et al.
Aging, 12(16), 15906-15917 (2020-08-30)
Telomeres are transcribed into long non-coding RNAs known as Telomeric Repeat-Containing RNA (TERRA). They have been shown to be essential regulators of telomeres and to act as epigenomic modulators at extra-telomeric sites. However the role of TERRA during early embryonic
Manami Kobayashi et al.
Frontiers in cell and developmental biology, 9, 753642-753642 (2021-11-26)
Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to
Faiza Basheer et al.
Bio-protocol, 10(5), e3536-e3536 (2021-03-05)
The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection.
Regina A Easley et al.
Environmental science & technology, 46(9), 5018-5024 (2012-04-03)
This work examines the use of purified meta-cresol purple (mCP) for direct spectrophotometric calibration of glass pH electrodes in seawater. The procedures used in this investigation allow for simple, inexpensive electrode calibrations over salinities of 20-40 and temperatures of 278.15-308.15
Souvika Bakshi et al.
Plant cell reports, 31(6), 1093-1103 (2012-02-14)
A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a
S F Gabby Krens et al.
BMC genomics, 9, 196-196 (2008-04-30)
The MAPK signaling proteins are involved in many eukaryotic cellular processes and signaling networks. However, specific functions of most of these proteins in vertebrate development remain elusive because of potential redundancies. For instance, the upstream activation pathways for ERK1 and
Hyunku Shin et al.
Journal of nanoscience and nanotechnology, 13(11), 7287-7290 (2013-11-20)
Real-time detection of pH value in a living cell is in central importance for research about cells and diseases. In spite of the great advances in science and technology, pH measurement in a living cell is still limited in spatial
Fan Yang et al.
Cell death & disease, 10(6), 388-388 (2019-05-19)
CUL4A and CUL4B are closely related members in Cullin family and can each assemble a Cullin-RING E3 ligase complex (Cullin-RING Ligase 4A or 4B, CRL4A, or CRL4B) and participate in a variety of biological processes. Previously we showed that zebrafish
Alberto López-Miguel et al.
American journal of ophthalmology, 157(4), 788-798 (2014-01-15)
To determine if controlled environmental conditions can induce acute exacerbations of signs and symptoms in dry eye and asymptomatic subjects. Prospective cross-sectional study. Nineteen patients with dry eye and 20 asymptomatic controls were exposed to controlled low humidity (5% relative
Mihaela Necula et al.
The Journal of biological chemistry, 282(14), 10311-10324 (2007-02-08)
Alzheimer disease is characterized by the abnormal aggregation of amyloid beta peptide into extracellular fibrillar deposits known as amyloid plaques. Soluble oligomers have been observed at early time points preceding fibril formation, and these oligomers have been implicated as the
Pankaj Dixit et al.
Journal of pharmacological and toxicological methods, 65(1), 13-17 (2011-11-24)
Everted gut sac of rat intestine is a paradigm widely employed for determination of absorption kinetics of drugs along with evaluation of effects of absorption enhancers. Since its inception in 1954, it has been optimized to enhance tissue survival and
Mingming Zou et al.
Ultrasonics sonochemistry, 20(2), 685-695 (2012-11-17)
The interaction of Cresol Red derivatives (Cresol Red (o-Cresolsulfonphthalein), Cresol Red-DA (3,3'-Bis [N,N-di (carboxymethyl) aminomethyl]-o-cresolsulfonphthalein) and Cresol Red-DA-Fe(III) (3,3'-Bis [N,N-di (carboxymethyl) aminomethyl]-o-cresolsulfonphthalein-Ferrous(III)) with bovine serum albumin (BSA) were studied by the combination of ultraviolet spectroscopy, circular dichroism (CD) spectroscopy, fluorescence
Dong Yi et al.
Chembiochem : a European journal of chemical biology, 13(15), 2290-2300 (2012-09-25)
A pH-based high-throughput assay method has been developed for the rapid and reliable measurement of transketolase (TK) activity. The method is based on the decarboxylation of lithium hydroxypyruvate (HPA) as a hydroxyacetyl donor with an aldehyde acceptor, using phenol red
Shivshankar R Mane et al.
Small (Weinheim an der Bergstrasse, Germany), 14(36), e1801571-e1801571 (2018-08-07)
Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility
Martin R Silic et al.
Genetics, 215(4), 1067-1084 (2020-06-18)
The roles of bioelectric signaling in developmental patterning remain largely unknown, although recent work has implicated bioelectric signals in cellular processes such as proliferation and migration. Here, we report a mutation in the inwardly rectifying potassium channel (kir) gene, kcnj13/kir7.1
Naomi Nakagata
Methods in molecular biology (Clifton, N.J.), 693, 57-73 (2010-11-17)
Cryopreservation of mouse spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. Recently, we have established procedures for cryopreservation of mouse spermatozoa and in vitro fertilization using cryopreserved spermatozoa to obtain
Megan E Williamson et al.
PLoS genetics, 17(10), e1009792-e1009792 (2021-10-19)
The transformer (tra) gene is essential for female development in many insect species, including the Australian sheep blow fly, Lucilia cuprina. Sex-specific tra RNA splicing is controlled by Sex lethal (Sxl) in Drosophila melanogaster but is auto-regulated in L. cuprina.
Francisca Alcaraz-Pérez et al.
BMC biotechnology, 8, 81-81 (2008-10-29)
The dual-luciferase assay has been widely used in cell lines to determine rapidly but accurately the activity of a given promoter. Although this strategy has proved very useful, it does not allow the promoter and gene function to be analyzed
Doua Abdelrahman et al.
MethodsX, 8, 101418-101418 (2021-08-26)
Microinjection technique is one of the essential methodologies that are used widely in zebrafish research. Microinjection is utilized to perform genetic manipulations within the developing zebrafish model. Further, this technique is used to study a wide range of genetic diseases
Xu Liu et al.
PloS one, 8(4), e60189-e60189 (2013-04-06)
Phenol red is widely used in cell culture as a pH indicator. Recently, it also has been reported to have estrogen-like bioactivity and be capable of promoting cell proliferation in different cell lines. However, the effect of phenol red on
Seyed Mehdi Rajaei et al.
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians, 45(4), 825-829 (2015-01-31)
The objective of this study was to determine the aqueous fraction of the tear film using the phenol red thread test (PRTT) and paper point tear test (PPTT) in healthy adult European pond turtles (Emys orbicularis). Twenty-four healthy adult European
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