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P4707

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Feng Wu et al.
Communications biology, 4(1), 98-98 (2021-01-24)
G-quadruplex (G4) structures have been predicted in the genomes of many organisms and proven to play regulatory roles in diverse cellular activities. However, there is little information on the evolutionary history and distribution characteristics of G4s. Here, whole-genome characteristics of...
Germana Rappa et al.
Oncotarget, 6(10), 7970-7991 (2015-03-13)
Interaction of breast cancer cells (BCCs) with stromal components is critical for tumor growth and metastasis. Here, we assessed the role of CD9 in adhesion, migration and invasiveness of BCCs. We used co-cultures of BCCs and bone marrow-derived multipotent mesenchymal...
M A G Rabbani et al.
Journal of virology, 90(24), 11145-11156 (2016-11-01)
A major arm of cellular innate immunity is type I interferon (IFN), represented by IFN-α and IFN-β. Type I IFN transcriptionally induces a large number of cellular genes, collectively known as IFN-stimulated gene (ISG) proteins, which act as antivirals. The...
Ritika Tewari et al.
The Journal of biological chemistry, 296, 100311-100311 (2021-01-23)
ZAP-70 is a tyrosine kinase essential for T cell immune responses. Upon engagement of the T cell receptor (TCR), ZAP-70 is recruited to the specialized plasma membrane domains, becomes activated, and is released to phosphorylate its laterally segregated targets. A...
Tatjana Trcek et al.
Molecular cell, 78(5), 941-950 (2020-05-29)
mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism...
Joseph Mathew Kalappurakkal et al.
Cell, 177(7), 1738-1756 (2019-05-21)
Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are a major class of lipid-anchored plasma membrane proteins. GPI-APs form nanoclusters generated by cortical acto-myosin activity. While our understanding of the physical principles governing this process is emerging, the molecular machinery and functional relevance of GPI-AP...
Latha M Malaiyandi et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 32(6), 951-964 (2019-11-23)
The understanding of cellular Cd2+ accumulation and toxicity is hampered by a lack of fluorescent indicators selective for intracellular free Cd2+ ([Cd2+]i). In this study, we used depolarized MIN6 mouse pancreatic beta cells as a model for evaluating [Cd2+]i detection...
Mario Rodríguez-Arribas et al.
Journal of laboratory automation, 21(2), 246-259 (2015-08-26)
Most laboratories interested in autophagy use different imaging software for managing and analyzing heterogeneous parameters in immunofluorescence experiments (e.g., LC3-puncta quantification and determination of the number and size of lysosomes). One solution would be software that works on a user's...
Sergi Aranda et al.
Molecular and cellular biology, 28(19), 5899-5911 (2008-08-06)
Raf-MEK-extracellular signal-regulated kinase (Erk) signaling initiated by growth factor-engaged receptor tyrosine kinases (RTKs) is modulated by an intricate network of positive and negative feedback loops which determine the specificity and spatiotemporal characteristics of the intracellular signal. Well-known antagonists of RTK...
Justine D Mintern et al.
Autophagy, 11(6), 906-917 (2015-05-08)
Antigen-presenting cells survey their environment and present captured antigens bound to major histocompatibility complex (MHC) molecules. Formation of MHC-antigen complexes occurs in specialized compartments where multiple protein trafficking routes, still incompletely understood, converge. Autophagy is a route that enables the...
Stephane Gourguechon et al.
BMC cell biology, 10, 68-68 (2009-09-24)
The Trypanosoma brucei cell cycle is regulated by combinations of cyclin/CRKs (cdc2 related kinases). Recently, two additional cyclins (CYC10, CYC11) and six new CRK (CRK7-12) homologues were identified in the T. brucei genome database 12. Individual RNAi knockdowns of these...
Kai Yin et al.
Ecotoxicology and environmental safety, 194, 110412-110412 (2020-03-11)
Hydrogen sulfide (H2S) is a toxic air pollutant that causes immune damage. Recent studies have found that neutrophil extracellular trap (NET) formation is one way in which neutrophils exert immune functions. In addition, the formation of NETs is also related...
Gargi Roy et al.
mAbs, 10(3), 416-430 (2018-02-06)
The conserved glycosylation site Asn297 of a monoclonal antibody (mAb) can be decorated with a variety of sugars that can alter mAb pharmacokinetics and recruitment of effector proteins. Antibodies lacking the core fucose at Asn297 (afucosylated mAbs) show enhanced antibody-dependent...
Rik Mencke et al.
Cardiovascular research, 108(2), 220-231 (2015-06-28)
Cardiovascular disease (CVD) is the leading cause of death in patients with chronic kidney disease (CKD), a disease state that is strongly associated with loss of renal and systemic (alpha-)Klotho. Reversely, murine Klotho deficiency causes marked medial calcification. It is...
Alberto E Ayala-Sarmiento et al.
STAR protocols, 1(3), 100184-100184 (2020-12-31)
Mosaic analysis by dual recombinase-mediated cassette exchange (MADR) is a technology that allows stable and locus-specific integration of transgenic elements into recipient cells carrying loxP and FRT sites. Nevertheless, most cell lines lack these recombination-specific sites. This protocol describes a...
Dubhe B Bulté et al.
Biotechnology and bioengineering, 117(9), 2633-2647 (2020-05-22)
Chinese hamster ovary (CHO) cells are characterized by a low glucose catabolic efficiency, resulting in undesirable lactate production. Here, it is hypothesized that such low efficiency is determined by the transport of pyruvate into the mitochondria. The mitochondrial pyruvate carrier...
Baydaa El Shazely et al.
Insect biochemistry and molecular biology, 110, 60-68 (2019-05-06)
Antimicrobial peptides (AMPs) are important immune effectors in insects. Bacteria have a limited number of ways to resist AMPs, and AMP-resistance is often costly. Recently, it has become clear that AMP activities in vitro and in vivo differ. Although some...
Maarten M van Oene et al.
Scientific reports, 7, 43285-43285 (2017-03-08)
The bacterial flagellar motor of Escherichia coli is a nanoscale rotary engine essential for bacterial propulsion. Studies on the power output of single motors rely on the measurement of motor torque and rotation under external load. Here, we investigate the...
Nils J Lambacher et al.
Nature cell biology, 18(1), 122-131 (2015-11-26)
The transition zone (TZ) ciliary subcompartment is thought to control cilium composition and signalling by facilitating a protein diffusion barrier at the ciliary base. TZ defects cause ciliopathies such as Meckel-Gruber syndrome (MKS), nephronophthisis (NPHP) and Joubert syndrome (JBTS). However...
All polymer chip for amperometric studies of transmitter release from large groups of neuronal cells.
Larsen ST and Taboryski R
Analyst, 137, 5057-5057 (2012)
Christian Proepper et al.
PloS one, 6(11), e27045-e27045 (2011-11-22)
Abelson-interacting protein 1 (Abi-1) plays an important role for dendritic branching and synapse formation in the central nervous system. It is localized at the postsynaptic density (PSD) and rapidly translocates to the nucleus upon synaptic stimulation. At PSDs Abi-1 is...
Kashish Manchanda et al.
Arteriosclerosis, thrombosis, and vascular biology, 38(8), 1859-1867 (2018-06-16)
Objective- The leukocyte heme-enzyme MPO (myeloperoxidase) exerts proinflammatory effects on the vascular system primarily linked to its catalytic properties. Recent studies have shown that MPO, depending on its cationic charge, mediates neutrophil recruitment and activation. Here, we further investigated MPO's...
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Fangbin Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(44), 15752-15755 (2014-10-22)
Switching dynamics of flagellar motors of Escherichia coli is commonly observed through markers attached to the flagellar filaments. To eliminate possible complications resulting from the conformational transitions of these filaments and to look at the output of motors more directly...
Mari Sepp et al.
PloS one, 6(7), e22138-e22138 (2011-07-27)
Transcription factor 4 (TCF4 alias ITF2, E2-2, ME2 or SEF2) is a ubiquitous class A basic helix-loop-helix protein that binds to E-box DNA sequences (CANNTG). While involved in the development and functioning of many different cell types, recent studies point...
Sayantani Sinha et al.
STAR protocols, 1(3), 100161-100161 (2020-12-31)
We describe a protocol for a long-term co-culture assay to study the contribution of mesenchymal stromal cells (MSCs) in regulating hematopoietic stem/progenitor cell (HSPC) activity. In addition, we describe the use of a clonogenic assay to determine myelo-erythroid differentiation. This...
You-Rong Lin et al.
Nanomaterials (Basel, Switzerland), 9(4) (2019-04-17)
Autophagy is the spontaneous degradation of intracellular proteins and organelles in response to nutrient deprivation. The phagocytosis of iron oxide nanoparticles (IONPs) results in intracellular degradation that can be exploited for use in cancer treatment. Non-invasive magnetic control has emerged...
Arkadiusz Urbański et al.
Micron (Oxford, England : 1993), 104, 8-20 (2017-10-20)
The evolutionary success of insects is undoubtedly related to a well-functioning immune system. This is especially apparent during insect development by the adaptation of individuals to the changing risk of infection. In addition, current studies show that the insect immune...
Nora C Goossen-Schmidt et al.
Scientific reports, 10(1), 13746-13746 (2020-08-15)
To suppress optical aberrations caused by refractive index mismatch, we employ glycerol-immersion objectives in conjunction with fused silica cover glasses and imaging buffers with a high glycerol content. Here we demonstrate that the addition of glycerol to the buffer does...
Shrawan Kumar Mishra et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864(2), 158-167 (2018-11-26)
Human GLTP on chromosome 12 (locus 12q24.11) encodes a 24 kD amphitropic lipid transfer protein (GLTP) that mediates glycosphingolipid (GSL) intermembrane trafficking and regulates GSL homeostatic levels within cells. Herein, we provide evidence that GLTP overexpression inhibits the growth of...
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