Eleftherios Michailidis et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1678-1688 (2020-01-10)
Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resistance to genetic manipulation have limited their use. Here, we show that the pyrrolizidine alkaloid retrorsine