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E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can
Aerosol-administered α-tocopherol attenuates lung inflammation in rats given lipopolysaccharide intratracheally.
Experimental Lung Research, 31, 34510-34524 (2007)
Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
Heat shock proteins expression during thermal risk exposure in the temperate xerothermic ant Formica cinerea
Slipinski P, et al.
Sociobiology, 62(3), 457-459 (2015)
Elena Kashuba et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(47), 19866-19871 (2009-11-12)
We report that the overexpression of mitochondrial ribosomal protein MRPS18-2 (S18-2) can immortalize primary rat embryonic fibroblasts (REFs). The immortalized cells (18IM) lose contact inhibition, form foci, and are capable of anchorage-independent growth. Concurrently, mesodermal markers, such as vimentin, smooth
JianFei Wang et al.
Journal of leukocyte biology, 82(6), 1554-1563 (2007-11-28)
Wound healing is a complex process involving the integrated actions of numerous cell types, soluble mediators, and ECM. Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic
Ondrej Hajdusek et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(4), 1033-1038 (2009-01-28)
Ticks are among the most important vectors of a wide range of human and animal diseases. During blood feeding, ticks are exposed to an enormous amount of free iron that must be appropriately used and detoxified. However, the mechanism of
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that
Julio Carrion et al.
Journal of immunology (Baltimore, Md. : 1950), 189(6), 3178-3187 (2012-08-15)
The low-grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. In this study, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic
Samuel Plante et al.
Genetics, 211(3), 893-911 (2019-01-17)
Spore germination is a process whereby spores exit dormancy to become competent for mitotic cell division. In Schizosaccharomyces pombe, one critical step of germination is the formation of a germ tube that hatches out the spore wall in a stage
Swatismita Ray et al.
Transgenic research, 21(2), 351-366 (2011-07-05)
Two abiotic stress-inducible upstream regulatory sequences (URSs) from rice have been identified and functionally characterized in rice. NBS-Str1 and BLEC-Str8 genes have been identified, by analysing the transcriptome data of cold, salt and desiccation stress-treated 7-day-old rice (Oryza sativa L.
Guohong Mao et al.
The Journal of biological chemistry, 286(9), 7548-7557 (2010-12-30)
Sulfurtransferases (STRs) catalyze the transfer of a sulfur atom from a donor to a suitable acceptor molecule. The Arabidopsis thaliana genome encodes 20 putative STR proteins. The biological functions of most are unclear. We found that STR1 and STR2 play
Liuqing Yang et al.
Chemistry & biodiversity, 7(4), 860-870 (2010-04-17)
Strictosidine synthase (STR1) catalyzes the stereoselective formation of 3alpha(S)-strictosidine from tryptamine and secologanin. Strictosidine is the key intermediate in the biosynthesis of 2,000 plant monoterpenoid indole alkaloids, and it is a key precursor of enzyme-mediated synthesis of alkaloids. An improved
Joachim Stockigt et al.
Natural product research, 24(8), 759-766 (2010-05-01)
A dual vector (pQE-70-STR1-SG) containing coding regions of strictosidine synthase (STR1, EC 4.3.3.2) and strictosidine glucosidase (SG, EC 3.2.1.105) from the Indian medicinal plant Rauvolfia serpentina was constructed. Functional expression of the vector in Escherichia coli cells (M15 strain) was
M D Sternlicht et al.
Cell, 98(2), 137-146 (1999-07-31)
Matrix metalloproteinases (MMPs) are invariably upregulated in the stromal compartment of epithelial cancers and appear to promote invasion and metastasis. Here we report that phenotypically normal mammary epithelial cells with tetracycline-regulated expression of MMP3/stromelysin-1 (Str1) form epithelial glandular structures in
Speranza Rubattu et al.
Journal of the American Heart Association, 5(2) (2016-02-19)
The genetic basis of stroke susceptibility remains to be elucidated. STR1 quantitative trait locus (STR1/QTL) was identified on rat chromosome 1 of stroke-prone spontaneously hypertensive rat (SHRSP) upon Japanese-style stroke-permissive diet (JD), and it contributes to 20% of the stroke
Fangrui Wu et al.
Journal of the American Chemical Society, 134(3), 1498-1500 (2012-01-11)
The Pictet-Spenglerase strictosidine synthase (STR1) has been recognized as a key enzyme in the biosynthesis of some 2000 indole alkaloids in plants, some with high therapeutic value. In this study, a novel function of STR1 has been detected which allows
Speranza Rubattu et al.
Journal of the American Heart Association, 5(2) (2016-02-19)
The genetic basis of stroke susceptibility remains to be elucidated. STR1 quantitative trait locus (STR1/QTL) was identified on rat chromosome 1 of stroke-prone spontaneously hypertensive rat (SHRSP) upon Japanese-style stroke-permissive diet (JD), and it contributes to 20% of the stroke
S Rubattu et al.
Nature genetics, 13(4), 429-434 (1996-08-01)
Stroke is a complex disorder with a poorly understood multifactorial and polygenic aetiology. We used the stroke-prone spontaneously hypertensive rat (SHRSP) as a model organism, mated it with the stroke-resistant spontaneously hypertensive rat (SHR) and performed a genome-wide screen in
Martin de Boer et al.
Blood cells, molecules & diseases, 55(4), 320-327 (2015-10-16)
Chronic granulomatous disease (CGD) is a rare congenital immune deficiency caused by mutations in any of the five genes encoding NADPH oxidase subunits. One of these genes is NCF1, encoding the p47(phox) protein. A group of 39 patients, 14 of
Chen Lv et al.
International journal of clinical and experimental medicine, 8(5), 7152-7159 (2015-07-30)
Neuropathic pain is caused by lesion or inflammation of the nervous system and characterized by the symptoms of allodynia, hyperalgesia and spontaneous pain. SIRT1 (Sir2) is a NAD-dependent deacetylase and is reported to regulate a wide variety of cellular processes
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