Showing 1-30 of 88 resultados for "T6258"
I O Daar et al.
Proceedings of the National Academy of Sciences of the United States of America, 83(20), 7903-7907 (1986-10-01)
Triose-phosphate isomerase (TPI; D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1) deficiency is a recessive disorder that results in hemolytic anemia and neuromuscular dysfunction. To determine the molecular basis of this disorder, a TPI allele from two unrelated patients homozygous for TPI deficiency was...
Viveka Mayya et al.
Science signaling, 2(84), ra46-ra46 (2009-08-20)
Protein phosphorylation events during T cell receptor (TCR) signaling control the formation of complexes among proteins proximal to the TCR, the activation of kinase cascades, and the activation of transcription factors; however, the mode and extent of the influence of...
Feifei Ren et al.
Chembiochem : a European journal of chemical biology, 14(2), 195-199 (2013-01-17)
Well structured: As a new triose phosphate isomerase (TIM) barrel-fold prenyl transferase, PcrB catalyzes the production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate. Crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus in complex with ligands were solved...
S C Mande et al.
Protein science : a publication of the Protein Society, 3(5), 810-821 (1994-05-01)
The crystal structure of recombinant human triosephosphate isomerase (hTIM) has been determined complexed with the transition-state analogue 2-phosphoglycolate at a resolution of 2.8 A. After refinement, the R-factor is 16.7% with good geometry. The asymmetric unit contains 1 complete dimer...
Rebecca Blomberg et al.
Nature, 503(7476), 418-421 (2013-10-18)
Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of...
Wei Li et al.
Experimental parasitology, 135(2), 223-226 (2013-07-31)
To assess the potential zoonotic transmission of giardiasis from dogs in China, a total of 205 fecal specimens from dogs were screened for Giardia duodenalis using PCR and sequence analysis of the triosephosphate isomerase gene. The prevalence of G. duodenalis...
F Di Piazza et al.
Journal of preventive medicine and hygiene, 54(4), 212-217 (2014-05-02)
A cross-sectional study was undertaken to determine the prevalence of Giardia and Cryptosporidium in calves of Palermo area (Sicily) and to evaluate the occupational risk associated with occurrence of zoonotic genotypes. A total of 217 faecal samples, from 149 calves...
Brandy J Johnson et al.
PloS one, 7(11), e50356-e50356 (2012-12-12)
The potential for encapsulating RNA within tunable, semi-permeable structures for storage and transportation purposes offers an interesting approach to the reduction of stringent storage requirements that often hamper the field application of genetic analysis methods. In this study, we assessed...
Weizhe Zhang et al.
PLoS neglected tropical diseases, 6(9), e1826-e1826 (2012-10-03)
Giardia duodenalis is a widespread intestinal protozoan of both humans and mammals. To date, few epidemiological studies have assessed the potential and importance of zoonotic transmission; and the human giardiasis burden attributable to G. duodenalis of animal origin is unclear....
Andrea V Scorza et al.
Veterinary parasitology, 189(2-4), 182-188 (2012-06-02)
The objective of this study was to determine and compare the assemblages of Giardia duodenalis isolated from mammalian fecal samples using the β-giardin (bg), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes. A total of 202 samples, either submitted to...
Javier Modrego et al.
European journal of nutrition, 52(2), 847-856 (2012-06-15)
Heart produces ATP through long-chain fatty acids beta oxidation. To analyze whether in ventricular myocardium, high-fat diet may modify the expression of proteins associated with energy metabolism before myocardial function was affected. Wistar Kyoto rats were divided into two groups:...
H Ji et al.
Electrophoresis, 18(3-4), 605-613 (1997-03-01)
The master two-dimensional gel database of human colon carcinoma cells currently lists cellular proteins from normal crypts and the colorectal cancer cell lines LIM 1863, LIM 1215 and LIM 1899 (Ward et al., Electrophoresis 1990, 11, 883-891; Ji et al....
Pramod K Kushawaha et al.
PloS one, 7(9), e45766-e45766 (2012-10-11)
Visceral leishmaniasis (VL) is one of the most important parasitic diseases with approximately 350 million people at risk. Due to the non availability of an ideal drug, development of a safe, effective, and affordable vaccine could be a solution for...
Noah Dephoure et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10762-10767 (2008-08-02)
The eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this...
Hak-Jae Kim et al.
Proteomics, 12(11), 1815-1829 (2012-05-25)
A decreased production of interferon gamma (IFNG) has been observed in acute schizophrenia. In order to explore the possible relationship between IFNG and schizophrenia, we attempted to analyze the differentially expressed proteins in the brains of interferon-gamma knockout (Ifng-KO) mice....
Eric M De Waal et al.
Biochemical and biophysical research communications, 434(4), 815-819 (2013-04-27)
The 'oxidative stress theory of aging' predicts that aging is primarily regulated by progressive accumulation of oxidized macromolecules that cause deleterious effects to cellular homeostasis and induces a decline in physiological function. However, our reports on the detection of higher...
Elisa E Figueroa-Angulo et al.
Parasitology, 139(13), 1729-1738 (2012-08-31)
The glycolytic enzyme triosephosphate isomerase catalyses the isomerization between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Here we report that Trichomonas vaginalis contains 2 fully functional tpi genes. Both genes are located in separated chromosomal context with different promoter regulatory elements and...
Daniela S Gerhard et al.
Genome research, 14(10B), 2121-2127 (2004-10-19)
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random...
Y Suzuki et al.
Gene, 200(1-2), 149-156 (1997-12-31)
Using 'oligo-capped' mRNA [Maruyama, K., Sugano, S., 1994. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 138, 171-174], whose cap structure was replaced by a synthetic oligonucleotide, we constructed two types of cDNA...
J R Brown et al.
Molecular and cellular biology, 5(7), 1694-1706 (1985-07-01)
The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase pairs and is split into seven exons. Its promoter contains putative TATA...
Ryan R Auld et al.
Journal of molecular evolution, 75(5-6), 198-213 (2012-11-28)
Isozymes, homologous enzymes coded by separate loci within a genome, present interesting systems for examining molecular and functional divergence through natural selection. Isozyme pairs for a number of metabolic enzymes, including Triosephosphate isomerase (Tpi), Malate dehydrogenase (Mdh), Phosphoglucose isomerase (Pgi)...
Red blood cell enzymopathies
Hematology, 616-625 (2018)
M Merced Malabanan et al.
Journal of the American Chemical Society, 134(24), 10286-10298 (2012-05-16)
The role of the hydrophobic side chains of Ile-172 and Leu-232 in catalysis of the reversible isomerization of R-glyceraldehyde 3-phosphate (GAP) to dihydroxyacetone phosphate (DHAP) by triosephosphate isomerase (TIM) from Trypanosoma brucei brucei (Tbb) has been investigated. The I172A and...
Bartholomew P Roland et al.
Biochimica et biophysica acta, 1852(1), 61-69 (2014-12-03)
Triosephosphate isomerase (TPI) is a glycolytic enzyme which homodimerizes for full catalytic activity. Mutations of the TPI gene elicit a disease known as TPI Deficiency, a glycolytic enzymopathy noted for its unique severity of neurological symptoms. Evidence suggests that TPI...
R K Rasmussen et al.
Electrophoresis, 18(3-4), 588-598 (1997-03-01)
MLK2, a member of the mixed lineage kinase (MLK) family of protein kinases, first reported by Dorow et al. (Eur. J. Biochem. 1993, 213, 701-710), comprises several distinct structural domains including an src homology-3 (SH3) domain, a kinase catalytic domain...
Li-Rong Yu et al.
Journal of proteome research, 6(11), 4150-4162 (2007-10-11)
Enrichment is essential for phosphoproteome analysis because phosphorylated proteins are usually present in cells in low abundance. Recently, titanium dioxide (TiO2) has been demonstrated to enrich phosphopeptides from simple peptide mixtures with high specificity; however, the technology has not been...
Montserrat Carrascal et al.
Journal of proteome research, 7(12), 5167-5176 (2009-04-16)
T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving...
Kristoffer T G Rigbolt et al.
Science signaling, 4(164), rs3-rs3 (2011-03-17)
To elucidate cellular events underlying the pluripotency of human embryonic stem cells (hESCs), we performed parallel quantitative proteomic and phosphoproteomic analyses of hESCs during differentiation initiated by a diacylglycerol analog or transfer to media that had not been conditioned by...
Sonia Dorion et al.
Planta, 236(4), 1177-1190 (2012-06-09)
Triosephosphate isomerase (TPI, EC 5.3.1.1) catalyzes the interconversion of dihydroxyacetone-P and glyceraldehyde 3-P in the glycolytic pathway. A constitutively expressed antisense construct for cytosolic TPI was introduced into potato (Solanum tuberosum) using Agrobacterium rhizogenes to examine the metabolic effects of...
Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet
Modrego, J., et al.
European Journal of Nutrition, DOI: 10-DOI: 10 (2012)

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