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WTA1

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Christine M Malboeuf et al.
Nucleic acids research, 41(1), e13-e13 (2012-09-11)
RNA viruses are the causative agents for AIDS, influenza, SARS, and other serious health threats. Development of rapid and broadly applicable methods for complete viral genome sequencing is highly desirable to fully understand all aspects of these infectious agents as
Anne H Rowley et al.
The Journal of infectious diseases, 203(7), 1021-1030 (2011-03-16)
Intracytoplasmic inclusion bodies (ICI) have been identified in ciliated bronchial epithelium of Kawasaki disease (KD) patients using a synthetic antibody derived from acute KD arterial IgA plasma cells; ICI may derive from the KD etiologic agent. Acute KD bronchial epithelium
J K Parker et al.
Journal of applied microbiology, 111(1), 216-223 (2011-04-12)
Viral pathogens in environmental media are generally highly diffuse, yet small quantities of pathogens may pose a health risk. This study evaluates the ability of TransPlex™ whole transcriptome amplification (WTA) to amplify small quantities of RNA viruses from complex environmental
Dongjun Lee et al.
Stem cells (Dayton, Ohio), 29(3), 539-548 (2011-03-23)
The Ets transcription factor Er71 is an important regulator of endothelial and hematopoietic development during mammalian embryogenesis. However, the role of Er71 in adult hematopoiesis has remained unknown. We now first show that conditional deletion of Er71 in the hematopoietic
Ana Sílvia Pires-Luís et al.
Epigenetics, 10(11), 1033-1043 (2015-10-22)
Renal cell tumors (RCTs) are the most lethal of the common urological cancers. The widespread use of imaging entailed an increased detection of small renal masses, emphasizing the need for accurate distinction between benign and malignant RCTs, which is critical
Elzbieta Brzuszkiewicz et al.
PloS one, 6(6), e21581-e21581 (2011-07-09)
The anaerobic gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance
Kinjal D Patel et al.
Experimental and molecular pathology, 113, 104378-104378 (2020-01-14)
High recurrence and poor overall survival in buccal mucosa squamous cell carcinoma (BMSCC) are not well addressed due to lack of efficient prognostic biomarkers and targeted therapies. To uncover gene candidates for the same, transcriptome profiling has been examined in
Sanela Svraka et al.
The Journal of general virology, 91(Pt 11), 2846-2856 (2010-07-28)
The use of metagenomics for virus discovery in clinical samples has opened new opportunities for understanding the aetiology of unexplained illness. This study explores the potential of this sequence-independent approach in a public-health setting, by systematic analysis of samples cultured
Eszter Posfai et al.
Genes & development, 26(9), 920-932 (2012-04-14)
In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei
Ki Wook Kim et al.
Open forum infectious diseases, 6(2), ofz025-ofz025 (2019-03-01)
The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture
Maternal cholestasis during pregnancy programs metabolic disease in offspring
Papacleovoulou G, et al.
Investigacion Clinica, doi:10-doi:10 (2013)
Sara Planamente et al.
Molecular microbiology, 86(5), 1085-1099 (2012-10-10)
GABA acts as an intercellular signal in eukaryotes and as an interspecies signal in host-microbe interactions. Structural characteristics of selective eukaryotic GABA receptors and bacterial GABA sensors are unknown. Here, we identified the selective GABA-binding protein, called Atu4243, in the
Shihori Yokobayashi et al.
Nature, 495(7440), 236-240 (2013-03-15)
In mammals, sex differentiation of primordial germ cells (PGCs) is determined by extrinsic cues from the environment. In mouse female PGCs, expression of stimulated by retinoic acid gene 8 (Stra8) and meiosis are induced in response to retinoic acid provided
Norman N Iscove et al.
Nature biotechnology, 20(9), 940-943 (2002-08-13)
Analysis of transcript representation on gene microarrays requires microgram amounts of total RNA or DNA. Without amplification, such amounts are obtainable only from millions of cells. However, it may be desirable to determine transcript representation in few or even single
Phuong-Nam Nguyen et al.
European urology, 59(3), 407-414 (2010-12-15)
The TMPRSS2:ERG fusion is both prevalent and unique to prostate cancer (PCa) and has great potential for noninvasive diagnosis of PCa in bodily fluids. To evaluate the specificity and sensitivity of the TMPRSS2:ERG fusion in urine from diverse clinical contexts
Munehiro Okamoto et al.
Scientific reports, 5, 8850-8850 (2015-03-07)
We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously
B A Benayoun et al.
Oncogene, 32(22), 2739-2746 (2012-07-17)
Ovarian granulosa cell tumors (OGCT) are the most frequent kind of sex cord-stromal tumors, and represent ∼2-5% of all ovarian malignancies. OGCTs exist as two entities, juvenile and adult types, with specific clinical and pathological characteristics. The molecular pathogenesis of
Catarina Guimarães-Teixeira et al.
Journal of personalized medicine, 11(10) (2021-10-24)
(1) Background: Methylation of N6-adenosine (m6A) is the most abundant messenger RNA (mRNA) modification in eukaryotes. We assessed the expression profiles of m6A regulatory proteins in renal cell carcinoma (RCC) and their clinical relevance, namely, as potential biomarkers. (2) Methods:
Ken Cadwell et al.
Cell, 141(7), 1135-1145 (2010-07-07)
It is unclear why disease occurs in only a small proportion of persons carrying common risk alleles of disease susceptibility genes. Here we demonstrate that an interaction between a specific virus infection and a mutation in the Crohn's disease susceptibility
Sa Xiao et al.
Virus research, 145(1), 80-91 (2009-06-23)
The complete genome sequence of avian paramyxovirus serotype 7 (APMV-7) prototype strain dove/Tennessee/4/75 was determined. The genome size is 15,480 nucleotides (nt) long and follows the "rule of six". The genome contains six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5'.
Michael C Risk et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 16(22), 5414-5423 (2010-10-12)
Several malignancies are known to exhibit a "field effect," whereby regions beyond tumor boundaries harbor histologic or molecular changes that are associated with cancer. We sought to determine if histologically benign prostate epithelium collected from men with prostate cancer exhibits
Sandra Klur et al.
Genomics, 83(3), 508-517 (2004-02-14)
Various approaches have been developed for the preparation of samples for gene expression monitoring. For Affymetrix chips, a standard protocol is widely used; however, this is inefficient for small samples such as laser capture microdissections. Several amplification procedures for such
Robert Schmieder et al.
BMC bioinformatics, 11, 341-341 (2010-06-25)
Sequencing metagenomes that were pre-amplified with primer-based methods requires the removal of the additional tag sequences from the datasets. The sequenced reads can contain deletions or insertions due to sequencing limitations, and the primer sequence may contain ambiguous bases. Furthermore
Eva Gonzalez-Roca et al.
PloS one, 5(12), e14418-e14418 (2011-01-05)
Expression profiling, the measurement of all transcripts of a cell or tissue type, is currently the most comprehensive method to describe their physiological states. Given that accurate profiling methods currently available require RNA amounts found in thousands to millions of
Omaththage P Perera et al.
PloS one, 10(6), e0128563-e0128563 (2015-06-06)
Heliothine pests such as the tobacco budworm, Heliothis virescens (F.), pose a significant threat to production of a variety of crops and ornamental plants and are models for developmental and physiological studies. The efforts to develop new control measures for
Xinghua Pan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(2), 594-599 (2012-12-26)
The ability to determine the gene expression pattern in low quantities of cells or single cells is important for resolving a variety of problems in many biological disciplines. A robust description of the expression signature of a single cell requires
Bridget S Banach et al.
Journal of virological methods, 156(1-2), 19-26 (2008-11-26)
Propagation of new human respiratory virus pathogens in established cell lines is hampered by a lack of predictability regarding cell line permissivity and by availability of suitable antibody reagents to detect infection in cell lines that do not exhibit significant
Ki Wook Kim et al.
Pediatric diabetes, 21(2), 271-279 (2019-12-05)
Microbial exposures in utero and early life shape the infant microbiome, which can profoundly impact on health. Compared to the bacterial microbiome, very little is known about the virome. We set out to characterize longitudinal changes in the gut virome
M Hertzberg et al.
The Plant journal : for cell and molecular biology, 25(5), 585-591 (2001-04-20)
Microarray technology is becoming an important comprehensive tool to study gene expression in plants. However, the use of this technology is limited by the large amount of sample tissue needed for microarray analysis. Generally, 50-200 microg of total RNA and
Madeline Vargas et al.
mBio, 4(2), e00105-e00113 (2013-03-14)
It has been proposed that Geobacter sulfurreducens requires conductive pili for long-range electron transport to Fe(III) oxides and for high-density current production in microbial fuel cells. In order to investigate this further, we constructed a strain of G. sulfurreducens, designated
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