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Anna Sheveleva et al.
Virus genes, 47(2), 385-388 (2013-07-03)
The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using
Luis León-Mateos et al.
Journal of clinical medicine, 9(7) (2020-07-08)
Current therapeutic options in the course of metastatic castration-resistant prostate cancers (mCRPC) reinforce the need for reliable tools to characterize the tumor in a dynamic way. Circulating tumor cells (CTCs) have emerged as a viable solution to the problem, whereby
Sebastiaan Theuns et al.
Genome announcements, 3(3) (2015-05-23)
Porcine epidemic diarrhea virus (PEDV) is a member of the family Coronaviridae and can cause severe outbreaks of diarrhea in piglets from different age groups. Here, we report the complete genome sequence (28,028 nt) of a PEDV strain isolated during a
Lee-Ann Tjon-Kon-Fat et al.
The Prostate, 78(1), 48-53 (2017-11-03)
Novel therapies for castration resistant prostate cancer (CRPC) have been introduced in the clinic with possibilities for individualized treatment plans. Best practice of those expensive drugs requires predictive biomarker monitoring. This study used circulating biomarker analysis to follow cancer-derived transcripts
Hanène Badri et al.
MicrobiologyOpen, 4(2), 187-207 (2015-02-14)
The aim of this work was to characterize in detail the response of Arthrospira to ionizing radiation, to better understand its radiation resistance capacity. Live cells of Arthrospira sp. PCC 8005 were irradiated with 60 Co gamma rays. This study
Lindsey B Turnbull et al.
PloS one, 12(11), e0187595-e0187595 (2017-11-08)
Gene expression DNA microarrays have been vital for characterizing whole-genome transcriptional profiles. Nevertheless, their effectiveness relies heavily on the accuracy of genome sequences, the annotation of gene structures, and the sequence-dependent performance of individual probes. Currently available gene expression arrays
Richard J Hall et al.
Journal of virological methods, 195, 194-204 (2013-09-17)
The discovery of new or divergent viruses using metagenomics and high-throughput sequencing has become more commonplace. The preparation of a sample is known to have an effect on the representation of virus sequences within the metagenomic dataset yet comparatively little
J K Parker et al.
Journal of applied microbiology, 111(1), 216-223 (2011-04-12)
Viral pathogens in environmental media are generally highly diffuse, yet small quantities of pathogens may pose a health risk. This study evaluates the ability of TransPlex™ whole transcriptome amplification (WTA) to amplify small quantities of RNA viruses from complex environmental
Marco Pellino et al.
Genes, 11(7) (2020-07-08)
Apomixis, the asexual reproduction via seeds, is associated to polyploidy and hybridization. To identify possible signatures of apomixis, and possible candidate genes underlying the shift from sex to apomixis, microarray-based gene expression patterns of live microdissected ovules at four different
Sanela Svraka et al.
The Journal of general virology, 91(Pt 11), 2846-2856 (2010-07-28)
The use of metagenomics for virus discovery in clinical samples has opened new opportunities for understanding the aetiology of unexplained illness. This study explores the potential of this sequence-independent approach in a public-health setting, by systematic analysis of samples cultured
Nana Owusu-Boaitey et al.
Immunity, inflammation and disease, 4(4), 413-426 (2016-12-17)
Uropathogenic Escherichia coli (UPEC), the causative agent of over 85% of urinary tract infections (UTIs), elaborate a number of siderophores to chelate iron from the host. On the other hand, the host immune imperative is to limit the availability of
Elzbieta Brzuszkiewicz et al.
PloS one, 6(6), e21581-e21581 (2011-07-09)
The anaerobic gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance
Csaba Vastagh et al.
Neuroendocrinology, 102(1-2), 44-59 (2015-05-01)
Gonadotropin-releasing hormone (GnRH) neurons play a pivotal role in the regulation of the hypothalamic-pituitary gonadal axis in a sex-specific manner. We hypothesized that the differences seen in reproductive functions of males and females are associated with a sexually dimorphic gene
Madapura M Pradeepa et al.
Nucleic acids research, 42(14), 9021-9032 (2014-07-25)
Trithorax and polycomb group proteins are generally thought to antagonize one another. The trithorax family member MLL (myeloid/lymphoid or mixed-lineage leukemia) is presumed to activate Hox expression, counteracting polycomb-mediated repression. PC4 and SF2 interacting protein 1 (PSIP1)/p75, also known as
Elena V Poddubskaya et al.
Experimental hematology & oncology, 7, 21-21 (2018-09-12)
Cholangiocarcinoma is an aggressive tumor with poor prognosis. Most of the cases are not available for surgery at the stage of the diagnosis and the best clinical practice chemotherapy results in about 12-month median survival. Several tyrosine kinase inhibitors (TKIs)
Jessy Vibin et al.
Scientific reports, 8(1), 8686-8686 (2018-06-08)
We present an optimised metagenomics method for detection and characterisation of all virus types including single and double stranded DNA/RNA and enveloped and non-enveloped viruses. Initial evaluation included both spiked and non-spiked bird faecal samples as well as non-spiked human
Akiko Satoh et al.
Aging cell, 14(2), 209-218 (2014-12-30)
The dorsomedial hypothalamus (DMH) controls a number of essential physiological responses. We have demonstrated that the DMH plays an important role in the regulation of mammalian aging and longevity. To further dissect the molecular basis of the DMH function, we
Yongqiang Zhou et al.
BioMed research international, 2017, 2512536-2512536 (2017-03-16)
This study aims to evaluate the effectiveness and clinical performance of a panel of urinary biomarkers to diagnose prostate cancer (PCa) in Chinese men with PSA levels between 4 and 10 ng/mL. A total of 122 patients with PSA levels between
Roger B Deal et al.
Nature protocols, 6(1), 56-68 (2011-01-08)
Genomic studies of cell differentiation and function within a whole organism depend on the ability to isolate specific cell types from a tissue, but this is technically difficult. We developed a method called INTACT (isolation of nuclei tagged in specific
Gene expression of benthic amphipods (genus:
Kalia S I Bistolas et al.
PeerJ, 5, e3810-e3810 (2017-10-03)
Maternal cholestasis during pregnancy programs metabolic disease in offspring
Papacleovoulou G, et al.
Investigacion Clinica, doi:10-doi:10 (2013)
Zahedan rhabdovirus, a novel virus detected in ticks from Iran
Dilcher M, et al
Virology Journal, 12, 183-183 (2015)
Martin T Jahn et al.
Cell host & microbe, 26(4), 542-550 (2019-09-29)
Phages are increasingly recognized as important members of host-associated microbiomes, with a vast genomic diversity. The new frontier is to understand how phages may affect higher order processes, such as in the context of host-microbe interactions. Here, we use marine
Luiz F Lisboa et al.
Canadian journal of gastroenterology = Journal canadien de gastroenterologie, 26(11), 799-805 (2012-11-21)
The molecular adsorbent recirculating system (MARS) is an albumin-dialysis modality that has been investigated predominantly in patients with acute and acute-on-chronic liver failure. To report the clinical efficacy and safety of MARS therapy for intractable pruritus in cholestasis patients with
Adrienne M S Correa et al.
The ISME journal, 7(1), 13-27 (2012-07-14)
The residence of dinoflagellate algae (genus: Symbiodinium) within scleractinian corals is critical to the construction and persistence of tropical reefs. In recent decades, however, acute and chronic environmental stressors have frequently destabilized this symbiosis, ultimately leading to coral mortality and
James M Flynn et al.
Journal of visualized experiments : JoVE, (58)(58), e3302-e3302 (2012-01-11)
While numerous studies have examined gene expression changes from homogenates of heart tissue, this prevents studying the inherent stochastic variation between cells within a tissue. Isolation of pure cardiomyocyte populations through a collagenase perfusion of mouse hearts facilitates the generation
C De Paepe et al.
Molecular reproduction and development, 86(1), 53-62 (2018-10-30)
Comprehensive understanding of lineage differentiation and apoptosis processes is important to increase our knowledge of human preimplantation development in vitro. We know that BMP signaling is important for different processes during mammalian development. In mouse preimplantation embryos, BMP signaling has
Distinct degenerative phenotype of articular cartilage from knees with meniscus tear compared to knees with osteoarthritis
Rai MF, et al.
Osteoarthritis and Cartilage, 27, 945-955 (2019)
Sam W Chan et al.
Cancer medicine, 2(1), 63-75 (2013-10-18)
We explore noninvasive clinical applications of multiple disease-specific fusion markers recently discovered in prostate cancer to predict the risk of cancer occurrence and aggressiveness at diagnosis. A total of 92 men who were prostate-specific antigen (PSA) screened and scheduled for
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