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Xenotransplanted Human Cortical Neurons Reveal Species-Specific Development and Functional Integration into Mouse Visual Circuits.

Neuron (2019-11-26)
Daniele Linaro, Ben Vermaercke, Ryohei Iwata, Arjun Ramaswamy, Baptiste Libé-Philippot, Leila Boubakar, Brittany A Davis, Keimpe Wierda, Kristofer Davie, Suresh Poovathingal, Pier-Andrée Penttila, Angéline Bilheu, Lore De Bruyne, David Gall, Karl-Klaus Conzelmann, Vincent Bonin, Pierre Vanderhaeghen
RESUMEN

How neural circuits develop in the human brain has remained almost impossible to study at the neuronal level. Here, we investigate human cortical neuron development, plasticity, and function using a mouse/human chimera model in which xenotransplanted human cortical pyramidal neurons integrate as single cells into the mouse cortex. Combined neuronal tracing, electrophysiology, and in vivo structural and functional imaging of the transplanted cells reveal a coordinated developmental roadmap recapitulating key milestones of human cortical neuron development. The human neurons display a prolonged developmental timeline, indicating the neuron-intrinsic retention of juvenile properties as an important component of human brain neoteny. Following maturation, human neurons in the visual cortex display tuned, decorrelated responses to visual stimuli, like mouse neurons, demonstrating their capacity for physiological synaptic integration in host cortical circuits. These findings provide new insights into human neuronal development and open novel avenues for the study of human neuronal function and disease. VIDEO ABSTRACT.

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Triton X-100, laboratory grade
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Magnesium chloride, anhydrous, ≥98%
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Magnesium sulfate, anhydrous, ReagentPlus®, ≥99.5%
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Potassium chloride, for molecular biology, ≥99.0%
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Guanosine 5′-triphosphate sodium salt hydrate, ≥95% (HPLC), powder
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Thiourea, ACS reagent, ≥99.0%
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Adenosine 5′-triphosphate magnesium salt, ≥95%, bacterial
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Dextrose, meets EP, BP, JP, USP testing specifications, anhydrous
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N-Methyl-D-glucamine, 99.0-100.5% (titration)
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