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  • Diverse Allyl Glucosinolate Catabolites Independently Influence Root Growth and Development.

Diverse Allyl Glucosinolate Catabolites Independently Influence Root Growth and Development.

Plant physiology (2020-04-24)
Ella Katz, Rammyani Bagchi, Verena Jeschke, Alycia R M Rasmussen, Aleshia Hopper, Meike Burow, Mark Estelle, Daniel J Kliebenstein
ABSTRACT

Glucosinolates (GSLs) are sulfur-containing defense metabolites produced in the Brassicales, including the model plant Arabidopsis (Arabidopsis thaliana). Previous work suggests that specific GSLs may function as signals to provide direct feedback regulation within the plant to calibrate defense and growth. These GSLs include allyl-GSL, a defense metabolite that is one of the most widespread GSLs in Brassicaceae and has also been associated with growth inhibition. Here we show that at least three separate potential catabolic products of allyl-GSL or closely related compounds affect growth and development by altering different mechanisms influencing plant development. Two of the catabolites, raphanusamic acid and 3-butenoic acid, differentially affect processes downstream of the auxin signaling cascade. Another catabolite, acrylic acid, affects meristem development by influencing the progression of the cell cycle. These independent signaling events propagated by the different catabolites enable the plant to execute a specific response that is optimal to any given environment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(−)-Sinigrin hydrate, from horseradish
Sigma-Aldrich
3-Butenoic acid, 97%
Millipore
Glutathione−Agarose, lyophilized powder
Sigma-Aldrich
Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Allyl cyanide, 98%
Sigma-Aldrich
(4R)-(−)-2-Thioxo-4-thiazolidinecarboxylic acid, 99%
Sigma-Aldrich
Allyl isothiocyanate, contains BHT as stabilizer, 95%
Sigma-Aldrich
Acrylic acid, anhydrous, contains 200 ppm MEHQ as inhibitor, 99%