Significant sensitivity improvements by matrix optimization: a MALDI-TOF mass spectrometric study of lipids from hen egg yolk.

Chemistry and physics of lipids (2010-04-28)
Kristin Teuber, Jürgen Schiller, Beate Fuchs, Michael Karas, Thorsten W Jaskolla
RESUMEN

Due to its sensitivity, the tolerance of impurities and the simplicity of performance, matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is increasingly used to analyze lipids from biological sources. Although its detailed role is not understood so far, the applied matrix has a pronounced effect on the achievable spectrum quality and particularly how sensitive the individual lipid classes are detectable. Different matrix compounds were recently established in the lipid field including 2,5-dihydroxybenzoic acid (DHB), 9-aminoacridine (9-AA), para-nitroaniline (PNA), 2-mercaptobenzothiazole (MBT), and 2-(2-aminoethylamino)-5-nitropyridine (AAN). It is the aim of this paper to compare the properties of these matrices with the newly synthesized matrix, alpha-cyano-2,4-difluorocinnamic acid (Di-FCCA). An organic extract from hen egg yolk was used as a simple and easily available test system. It will be shown that Di-FCCA is the matrix of choice to detect lipids in the positive-ion mode due to an achievable sensitivity gain of more than one order of magnitude compared to alternative matrices. In contrast, Di-FCCA is not suitable for negative-ion detection of phospholipids. Here, 9-AA is unequivocally the matrix of choice.

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Sigma-Aldrich
4-Nitroaniline, ≥99%
Sigma-Aldrich
2-Mercaptobenzothiazole, 97%
Supelco
4-Nitroaniline, analytical standard
Supelco
4-Chloro-α-cyanocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)
Sigma-Aldrich
2-Mercaptobenzothiazole, technical, ≥90% (T)
Supelco
α-Cyano-4-hydroxycinnamic acid - α-Cyano-2,4-difluorocinnamic acid - α-Cyano-2,3,4,5,6-pentafluorocinnamic acid mixture, matrix substance for MALDI-MS, ≥95.0% (sum of three components, HPLC)
Supelco
4-Bromo-α-cyanocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)
Supelco
α-Cyano-2,4-difluorocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)

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