[New opportunities of renal diseases diagnostics using gas chromatography].

Przeglad lekarski (2012-07-07)
Beata Grabowska-Polanowska, Jacek Faber, Monika Skowron, Przemysław Miarka, Agata Pietrzycka, Paweł Zagrodzki, Ireneusz Sliwka, Władysław Sutowicz, Marek Stepniewski
RESUMEN

During the last decade the amount of patients suffering from chronic kidney disease (CKD) has increased. The physicians' efforts are focused on early CKD diagnosis and reduction of the end-stage renal diseases incidence. The breath test seems to be a promising diagnostic device offering early noninvasive diseases detection. The aim of presented study was the determination of breath composition in case of persons suffering from CKD. Breath samples were collected from 14 patients and 7 healthy volunteers. Exhaled air samples were analyzed by gas chromatography equipped with mass spectrometer (GC-MS). Samples were enriched using solid phase microextraction (SPME). Trimethylamine (TMA), mentioned in literature as potential marker of chronic kidney diseases, was detected in case of 11 patients. Among breath components were detected: sulfur compounds: dimethyl sulfide (was detected in exhaled air of patients and control group), carbon disulfide (was detected in case of 4 healthy and in case of all patients) and also potential markers of oxidative stress: propane, butane, pentane, 2-methylpentane, hexane. Acetone and isoprene occurred in exhaled air of all studied persons. The considerable increase of acetone concentration in comparison to control group was observed in case of patients with diagnosed diabetes. The application of gas chromatography with mass spectrometer and appropriate enrichment of samples allows to define the breath profile characteristic for chosen the unit of disease. Typical compounds--biomarkers can be useful for early diagnostics.

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Carbon disulfide, anhydrous, ≥99%
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Carbon disulfide, ACS reagent, ≥99.9%
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Dimethyl sulfide, anhydrous, ≥99.0%
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Dimethyl sulfide, ≥99%
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Trimethylamine solution, 43.0-49.0% in H2O (T)
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Trimethylamine, anhydrous, ≥99%
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Trimethylamine hydrochloride, 98%
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Carbon disulfide, suitable for HPLC, ≥99.9%
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Dimethyl sulfide, redistilled, ≥99%, FCC, FG
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Trimethylamine solution, 31-35 wt. % in ethanol, 4.2 M, contains toluene as stabilizer
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Trimethylamine solution, 25 wt. % in H2O
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Dimethyl sulfide, natural, ≥99%, FCC, FG
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Dimethyl sulfide, analytical standard
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Trimethylamine solution, 25 wt. % in propylene glycol
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Carbon disulfide solution, 5 M in THF
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