Quantification of chemical elements in blood of patients affected by multiple sclerosis.

Annali dell'Istituto superiore di sanita (2005-10-26)
Giovanni Forte, Andrea Visconti, Simone Santucci, Anna Ghazaryan, Lorenzo Figà-Talamanca, Stefania Cannoni, Beatrice Bocca, Anna Pino, Nicola Violante, Alessandro Alimonti, Marco Salvetti, Giovanni Ristori
RESUMEN

Although some studies suggested a link between exposure to trace elements and development of multiple sclerosis (MS), clear information on their role in the aetiology of MS is still lacking. In this study the concentrations of Al, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn, Mo, Ni, Pb, Sb, Si, Sn, Sr, Tl, V, W, Zn and Zr were determined in the blood of 60 patients with MS and 60 controls. Quantifications were performed by inductively coupled plasma (ICP) atomic emission spectrometry and sector field ICP mass spectrometry. When the two groups were compared, an increased level of Co, Cu and Ni and a decrement of Be, Fe, Hg, Mg, Mo, Pb and Zn in blood of patients were observed. In addition, the discriminant analysis pointed out that Cu, Be, Hg, Co and Mo were able to discriminate between MS patients and controls (92.5% of cases correctly classified).

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Acetic acid, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
Sigma-Aldrich
L-Glutathione reduced, ≥98.0%
Sigma-Aldrich
L-Cysteine, 97%
Sigma-Aldrich
Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
Sigma-Aldrich
L-Cysteine, from non-animal source, BioReagent, suitable for cell culture, ≥98%
Sigma-Aldrich
L-Glutathione oxidized, ≥98% (HPLC)
Sigma-Aldrich
Zinc, dust, <10 μm, ≥98%
Sigma-Aldrich
Magnesium, powder, ≥99%
SAFC
L-Cysteine
Sigma-Aldrich
Magnesium, turnings, reagent grade, 98%
Sigma-Aldrich
L-Glutathione reduced, suitable for cell culture, BioReagent, ≥98.0%, powder
Sigma-Aldrich
Acetic acid, glacial, ≥99.99% trace metals basis
Sigma-Aldrich
Zinc, granular, 20-30 mesh, ACS reagent, ≥99.8%
Sigma-Aldrich
Acetic acid, natural, ≥99.5%, FG
Sigma-Aldrich
Zinc, purum, powder
Sigma-Aldrich
Acetic acid solution, suitable for HPLC
Sigma-Aldrich
Zinc, powder, <150 μm, 99.995% trace metals basis
Sigma-Aldrich
L-Cysteine, BioUltra, ≥98.5% (RT)
Supelco
Acetic acid, analytical standard
Sigma-Aldrich
Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2800, slurry, in H2O, active catalyst
Sigma-Aldrich
Calcium, granular, 99%
Sigma-Aldrich
Copper, powder, <425 μm, 99.5% trace metals basis
Sigma-Aldrich
L-Glutathione reduced, BioXtra, ≥98.0%
Sigma-Aldrich
Silicon, powder, −325 mesh, 99% trace metals basis
Sigma-Aldrich
Magnesium, purum, for Grignard reactions, ≥99.5%, turnings
Sigma-Aldrich
Manganese, powder, ≥99.9% trace metals basis
Sigma-Aldrich
Copper, foil, thickness 0.25 mm, 99.98% trace metals basis
Sigma-Aldrich
DL-Homocysteine, ≥95% (titration)