Merck

Phytochemical fingerprints of lime honey collected in serbia.

Journal of AOAC International (2015-04-24)
Uroš Gašić, Branko Šikoparija, Tomislav Tosti, Jelena Trifković, Dušanka Milojković-Opsenica, Maja Natić, Živoslav Tešić
RESUMEN

Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia. Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar. Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples. Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap). Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection. Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences. Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey. Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation. Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples. Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Isomaltose, ~98% (TLC)
Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
Millipore
Sucrose, ACS reagent, suitable for microbiology, ≥99.0%
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sigma-Aldrich
Maltose solution, BioReagent, for molecular biology, ~20% in H2O
Supelco
Acetonitrile, analytical standard
Supelco
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9% (GC)
Sigma-Aldrich
Naringin, ≥95% (HPLC)
Sigma-Aldrich
Naringin, ≥90%, from citrus fruit
Sigma-Aldrich
Sucrose, meets USP testing specifications
Sigma-Aldrich
Sucrose, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, Grade I, suitable for plant cell culture
Sigma-Aldrich
Sucrose, Grade II, suitable for plant cell culture
Sigma-Aldrich
Maltotriose, ≥90% (HPLC)
Sigma-Aldrich
Luteolin, ≥98% (TLC), powder
Sigma-Aldrich
Acetonitrile, ≥99.5%, ACS reagent
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, ACS reagent
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, ≥99.5%
Supelco
Sucrose, analytical standard, for enzymatic assay kit SCA20
Supelco
Chrysin, analytical standard
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
Formic acid, ≥95%, FCC, FG
Sigma-Aldrich
Chrysin, 97%
Supelco
Sucrose, analytical standard