• Inicio
  • Resultados de la búsqueda
  • Application of Optimized Vortex-Assisted Surfactant-Enhanced DLLME for Preconcentration of Thymol and Carvacrol, and Their Determination by HPLC-UV: Response Surface Methodology.

Application of Optimized Vortex-Assisted Surfactant-Enhanced DLLME for Preconcentration of Thymol and Carvacrol, and Their Determination by HPLC-UV: Response Surface Methodology.

Journal of chromatographic science (2015-02-01)
Mehrorang Ghaedi, Mostafa Roosta, Saeid Khodadoust, Ali Daneshfar
RESUMEN

A novel vortex-assisted surfactant-enhanced dispersive liquid-liquid microextraction combined with high-performance liquid chromatography (VASEDLLME-HPLC) was developed for the determination of thymol and carvacrol (phenolic compound). In this method, the extraction solvent (CHCl3) was dispersed into the aqueous samples via a vortex agitator and addition of the surfactant (Triton X-100). The preliminary experiments were undertaken to select the best extraction solvent and surfactant. The influences of effective variables were investigated using a Plackett-Burman 2(7-4) screening design and then, the significant variables were optimized by using a central composite design combined with desirability function. Working under optimum conditions specified as: 140 µL CHCl3, 0.08% (w/v, Triton X-100), 3 min extraction time, 6 min centrifugation at 4,500 rpm, pH 7, 0.0% (w/v) NaCl permit achievement of high and reasonable linear range over 0.005-4.0 mg L(-1) with R(2) = 0.9998 (n = 10). The separation of thymol and carvacrol was achieved in <14 min using a C18 column and an isocratic binary mobile phase acetonitrile-water (55:45, v/v) with a flow rate of 1.0 mL min(-1). The VASEDLLME is applied for successful determination of carvacrol and thymol in different thyme and pharmaceutical samples with relative standard deviation <4.7% (n = 5).

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
Sodium hydroxide, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
Sigma-Aldrich
Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
Sigma-Aldrich
Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
Sodium hydroxide, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
Sodium hydroxide solution, 50% in H2O
Sigma-Aldrich
Sodium dodecyl sulfate, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium hydroxide, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Sodium phosphate monobasic, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Phosphoric acid, 85 wt. % in H2O, 99.99% trace metals basis
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
Sigma-Aldrich
Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
Sigma-Aldrich
Sodium phosphate dibasic, ACS reagent, ≥99.0%
Sigma-Aldrich
Phosphoric acid, ACS reagent, ≥85 wt. % in H2O
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Sigma-Aldrich
Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H2O
Sigma-Aldrich
Sodium phosphate dibasic, BioXtra, ≥99.0%
Sigma-Aldrich
Sodium phosphate dibasic, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Sodium hydroxide, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets
Sigma-Aldrich
Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
Phosphoric acid solution, 85 wt. % in H2O, FCC, FG
Sigma-Aldrich
Triton X-114, laboratory grade
Sigma-Aldrich
Phosphoric acid, ACS reagent, ≥85 wt. % in H2O
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
Sigma-Aldrich
Thymol, ≥98.5%