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Effects of Enhanced UV-B Radiation on Biochemical Traits in Postharvest Flowers of Medicinal Chrysanthemum.

Photochemistry and photobiology (2015-03-18)
Chao Si, Xiao-Qin Yao, Xue-Li He, Jian-Zhou Chu, Chun-Hui Ma, Xiao-Fei Shi
ABSTRACT

This article reported UV-B radiation effects on biochemical traits in postharvest flowers of chrysanthemum. The experiment included six levels of UV-B radiation (UV0, 0 μW cm(-2); UV50, 50 μW cm(-2); UV200, 200 μW cm(-2); UV400, 400 μW cm(-2); UV600, 600 μW cm(-2) and UV800, 800 μW cm(-2). Enhanced UV-B radiation significantly increased hydrogen peroxide content (except for UV50), but did not evidently affect malondialdehyde content in flowers. Chlorophyll b and total chlorophyll content were significantly increased by UV600 and UV800. UV400 and UV600 significantly increased anthocyanins, carotenoids and UV-B absorbing compounds content, and the activities of phenylalanine ammonia lyase (PAL) and cinnamic acid-4-hydroxylase (C4H) over the control. 4-coumarate CoA ligase (4CL) activity was significantly decreased by enhanced UV-B radiation (except for UV50). The relationships between UV-B radiation intensities and the activities of secondary metabolism enzymes were best described by a second-order polynomial. The R(2) values for UV-B radiation intensities and the activities of PAL, C4H and 4CL were 0.8361, 0.5437 and 0.8025, respectively. The results indicated that enhanced UV-B radiation could promote secondary metabolism processes in postharvest flowers, which might be beneficial for the accumulation of medically active ingredients in medicinal plants. The optimal UV-B radiation intensities in the study were between UV400-UV600.

MATERIALS
Product Number
Brand
Product Description

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L-Phenylalanine, 99%, FCC
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trans-Cinnamic acid, ≥99%, FG
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L-Phenylalanine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
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trans-Cinnamic acid, ≥99%
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trans-Cinnamic acid, 97%
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