Merck

High-mobility group box-1 was released actively and involved in LPS induced depressive-like behavior.

Journal of psychiatric research (2015-03-22)
Teng-Yun Wu, Lei Liu, Wei Zhang, Yi Zhang, Yun-Zi Liu, Xiao-Liang Shen, Hong Gong, Yuan-Yuan Yang, Xiao-Ying Bi, Chun-Lei Jiang, Yun-Xia Wang
RESUMEN

Depression disorder is a common mental illness, of which the pathogenesis is not well understood. Studies suggest that immunity imbalance and up-regulation of pro-inflammatory cytokines may be associated with the pathogenesis of depression. High-mobility group box 1 protein (HMGB1) has gained much attention as an important player in innate immune responses and an modulating factor in several inflammatory diseases. Here we sought to explore the role of HMGB1 in the development of depression. Depression model was established with low dose of lipopolysaccharide (LPS) administration. Depressive behavior was reflected with increased immobility time in tail suspension test. Accompanying with depressive-like behavior, translocation of HMGB1 from nuclei to cytoplasm was observed by immunofluorescence assays. Meanwhile, no significant necrosis was observed evaluated by hematoxylin-eosin staining. These data indicated that HMGB1 was released actively in the central nervous system. In addition, treating the mice with human recombinant HMGB1 (rHMGB1) could induce the development of depressive-like behavior. Blockage of HMGB1 with GZA abrogated the depressive-like behavior induced by LPS or rHMGB1. These results implicated that HMGB1 was involved in LPS-induced depressive-like behavior.

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4′,6-Diamidino-2-phenylindole dihydrochloride, BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
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Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
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Glycyrrhizic acid ammonium salt from glycyrrhiza root (licorice), ≥95.0% (NT)
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4′,6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
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Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
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Glycyrrhizic acid ammonium salt from glycyrrhiza root (licorice), ≥70% (HPLC)
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Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
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HMG-1 human, lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli
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DAPI, for nucleic acid staining
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Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)