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  • Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions.

Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions.

Advanced materials (Deerfield Beach, Fla.) (2015-05-20)
Judith Guasch, Bert Conings, Stefanie Neubauer, Florian Rechenmacher, Karen Ende, Claudio G Rolli, Christian Kappel, Viktoria Schaufler, Alexandre Micoulet, Horst Kessler, Hans-Gerd Boyen, Elisabetta Ada Cavalcanti-Adam, Joachim P Spatz
RESUMEN

Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified αvβ3 and α5β1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such receptors. Moreover, this tendency can be altered by varying the geometry and composition of the micropatterns.

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