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Facultative Anaerobe Caldibacillus debilis GB1: Characterization and Use in a Designed Aerotolerant, Cellulose-Degrading Coculture with Clostridium thermocellum.

Applied and environmental microbiology (2015-06-07)
Scott Wushke, David B Levin, Nazim Cicek, Richard Sparling
RESUMEN

Development of a designed coculture that can achieve aerotolerant ethanogenic biofuel production from cellulose can reduce the costs of maintaining anaerobic conditions during industrial consolidated bioprocessing (CBP). To this end, a strain of Caldibacillus debilis isolated from an air-tolerant cellulolytic consortium which included a Clostridium thermocellum strain was characterized and compared with the C. debilis type strain. Characterization of isolate C. debilis GB1 and comparisons with the type strain of C. debilis revealed significant physiological differences, including (i) the absence of anaerobic metabolism in the type strain and (ii) different end product synthesis profiles under the experimental conditions used. The designed cocultures displayed unique responses to oxidative conditions, including an increase in lactate production. We show here that when the two species were cultured together, the noncellulolytic facultative anaerobe C. debilis GB1 provided respiratory protection for C. thermocellum, allowing the synergistic utilization of cellulose even under an aerobic atmosphere.

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Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
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Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
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Ethanol, anhydrous, denatured
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Sucrose, for molecular biology, ≥99.5% (GC)
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D-Sorbitol, ≥98%
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Sucrose, BioXtra, ≥99.5% (GC)
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Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
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Sucrose, ≥99.5%
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Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
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D-Sorbitol, 99%
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D-(+)-Mannose, powder, BioReagent, suitable for cell culture
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D-(−)-Ribose, ≥99%
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