Merck
  • Inicio
  • Resultados de la búsqueda
  • Rapid effects of essential fatty acid deficiency on growth and development parameters and transcription of key fatty acid metabolism genes in juvenile barramundi (Lates calcarifer).

Rapid effects of essential fatty acid deficiency on growth and development parameters and transcription of key fatty acid metabolism genes in juvenile barramundi (Lates calcarifer).

The British journal of nutrition (2015-09-29)
Michael J Salini, Giovanni M Turchini, Nicholas M Wade, Brett D Glencross
RESUMEN

Barramundi (Lates calcarifer), a catadromous teleost of significant and growing commercial importance, are reported to have limited fatty acid bioconversion capability and therefore require preformed long-chain PUFA (LC-PUFA) as dietary essential fatty acid (EFA). In this study, the response of juvenile barramundi (47·0 g/fish initial weight) fed isolipidic and isoenergetic diets with 8·2% added oil was tested. The experimental test diets were either devoid of fish oil (FO), and thus with no n-3 LC-PUFA (FO FREE diet), or with a low inclusion of FO (FO LOW diet). These were compared against a control diet containing only FO (FO CTRL diet) as the added lipid source, over an 8-week period. Interim samples and measurements were taken fortnightly during the trial in order to define the aetiology of the onset and progression of EFA deficiency. After 2 weeks, the fish fed the FO FREE and FO LOW diets had significantly lower live-weights, and after 8 weeks significant differences were detected for all performance parameters. The fish fed the FO FREE diet also had a significantly higher incidence of external abnormalities. The transcription of several genes involved in fatty acid metabolism was affected after 2 weeks of feeding, showing a rapid nutritional regulation. This experiment documents the aetiology of the onset and the progression of EFA deficiency in juvenile barramundi and demonstrates that such deficiencies can be detected within 2 weeks in juvenile fish.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Nitric acid, 70%, purified by redistillation, ≥99.999% trace metals basis
Sigma-Aldrich
Nitric acid, ACS reagent, ≥90.0%
Sigma-Aldrich
Nitric acid, puriss. p.a., 65.0-67.0%
Sigma-Aldrich
Nitric acid, puriss. p.a., ≥65% (T)
Sigma-Aldrich
Nitric acid, puriss. p.a., reag. ISO, reag. Ph. Eur., for determinations with dithizone, ≥65%
Sigma-Aldrich
Nitric acid, red, fuming, HNO3 >90 %
Sigma-Aldrich
Nitric acid, ACS reagent, 70%
Sigma-Aldrich
Yttrium, chips, 99.9% trace rare earth metals basis
Sigma-Aldrich
Yttrium, ingot, 99.9% trace rare earth metals basis
Sigma-Aldrich
Yttrium, powder, −40 mesh, 99.5% trace rare earth metals basis
Sigma-Aldrich
Nitric-14N acid solution, ~10 N in H2O, 99.99 atom % 14N
Yttrium, wire reel, 0.05m, diameter 0.5mm, 99.9%
Sigma-Aldrich
Yttrium sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.9% trace metals basis
Yttrium, foil, not light tested, 50x50mm, thickness 0.025mm, as rolled, 99%
Yttrium, foil, not light tested, 25x25mm, thickness 0.005mm, as rolled, 99%
Yttrium, foil, not light tested, 25x25mm, thickness 0.025mm, as rolled, 99%
Yttrium, rod, 100mm, diameter 6.35mm, cast, 99%
Yttrium, wire reel, 0.05m, diameter 1.0mm, 99.9%
Yttrium, wire reel, 0.05m, diameter 1.0mm, square section, 99.9%
Yttrium, wire reel, 0.1m, diameter 0.5mm, 99.9%
Yttrium, wire reel, 0.1m, diameter 1.0mm, 99.9%
Yttrium, wire reel, 0.2m, diameter 0.5mm, 99.9%
Yttrium, rod, 50mm, diameter 12.5mm, cast, 99%
Yttrium, rod, 50mm, diameter 6.35mm, cast, 99%
Yttrium, rod, 50mm, diameter 2.0mm, cast, 99%
Yttrium, wire reel, 0.2m, diameter 1.0mm, 99.9%
Yttrium, foil, 25mm disks, thickness 0.50mm, as rolled, 99%
Yttrium, foil, 50x50mm, thickness 0.15mm, as rolled, 99%
Yttrium, foil, 25x25mm, thickness 0.15mm, as rolled, 99%
Yttrium, foil, 50x50mm, thickness 0.125mm, as rolled, 99%