Merck
  • Home
  • Search Results
  • Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3.

Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3.

Archives of virology (2015-07-01)
Leonardo Joaquim van Zyl, Falone Sunda, Mark Paul Taylor, Don Arthur Cowan, Marla Iris Trindade
ABSTRACT

The study of extremophilic phages may reveal new phage families as well as different mechanisms of infection, propagation and lysis to those found in phages from temperate environments. We describe a novel siphovirus, GVE3, which infects the thermophile Geobacillus thermoglucosidasius. The genome size is 141,298 bp (G+C 29.6%), making it the largest Geobacillus spp-infecting phage known. GVE3 appears to be most closely related to the recently described Bacillus anthracis phage vB_BanS_Tsamsa, rather than Geobacillus-infecting phages described thus far. Tetranucleotide usage deviation analysis supports this relationship, showing that the GVE3 genome sequence correlates best with B. anthracis and Bacillus cereus genome sequences, rather than Geobacillus spp genome sequences.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3-Methylbutanol, BioReagent, for molecular biology, ≥98.5%
Sigma-Aldrich
Phenol, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
Sigma-Aldrich
S-Methyl methanethiosulfonate, 97%
Sigma-Aldrich
DL-Cysteine, technical grade
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H2O
Sigma-Aldrich
Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H2O
Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
Sigma-Aldrich
Phenol, ≥96.0% (calc. on dry substance, T)
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Sigma-Aldrich
Tetraethylammonium borohydride, technical, ≥95% (T)
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC), crystalline (detached)
Sigma-Aldrich
3-Methylbutanol, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
Isoamyl alcohol, ≥98%, FG
Sigma-Aldrich
Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Sigma-Aldrich
Phenol, for molecular biology
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
Sigma-Aldrich
Phenol, natural, 97%, FG
Sigma-Aldrich
Phenol, ≥99%
Sigma-Aldrich
Isoamyl alcohol, natural, ≥98%, FG
Sigma-Aldrich
Phenol solution, Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
Supelco
Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
3-Methyl-1-butanol, anhydrous, ≥99%