Photochemical coating of Kapton® with hydrophilic polymers for the improvement of neural implants.

Materials science & engineering. C, Materials for biological applications (2017-04-19)
Christoph Hadler, Kirsten Wissel, Gudrun Brandes, Wibke Dempwolf, Günter Reuter, Thomas Lenarz, Henning Menzel
RESUMEN

The polyimide Kapton® was coated photochemically with hydrophilic polymers to prevent undesirable cell growth on the polyimide surface. The polymer coatings were generated using photochemically reactive polymers synthesized by a simple and modular strategy. Suitable polymers or previously synthesized copolymer precursors were functionalized with photoactive arylazide groups by a polymer analogous amide coupling reaction with 4-azidobenzoic acid. A photoactive chitosan derivative (chitosan-Az) and photochemically reactive copolymers containing DMAA, DEAA or MTA as primary monomers were synthesized using this method. The amount of arylazide groups in the polymers was adjusted to approximately 5%, 10% and 20%. As coating on Kapton® all polymers effect a significantly reduced water contact angle (WCA) and consequently a rise of the surface hydrophilicity compared to the untreated Kapton®. The presence of the polymer coatings was also proven by ATR-IR spectroscopy. Coatings with chitosan-Az and the DEAA copolymer cause a distinct inhibition of the growth of fibroblasts. In the case of the DMAA copolymer even a strong anti-adhesive behavior towards fibroblasts was verified. Biocompatibility of the polymer coatings was proven which enables their utilization in biomedical applications.

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Sigma-Aldrich
2,2′-Azobis(2-methylpropionamidine) dihydrochloride, granular, 97%
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N,N-Dimethylacrylamide, 99%, contains 500 ppm monomethyl ether hydroquinone as inhibitor
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[2-(Methacryloyloxy)ethyl]trimethylammonium chloride solution, 75 wt. % in H2O
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N-(3-Aminopropyl)methacrylamide hydrochloride, contains ≤1,000 ppm MEHQ as stabilizer, 98% (HPLC)
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N,N-Dimethyldodecylamine, 97%
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N,N-Diethylacrylamide, contains <200 ppm MEHQ as inhibitor, 99%
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4-Azidobenzoic acid solution, ~0.2 M in tert-butyl methyl ether, ≥95.0% (HPLC)