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Based on the principle of adsorption/absorption and desorption, SPME uses a coated fiber to concentrate volatile and semi-volatile compounds from a sample. * * * ## Related Products Slide 1 of 16 1 of 4 [ \ Supelco \ 57330-U \ SPME Fiber Holder](https://www.sigmaaldrich.com/MY/en/product/supelco/57330u) Quick View [ \ Supelco \ 57347-U \ SPME Fiber Holder](https://www.sigmaaldrich.com/MY/en/product/supelco/57347u) Quick View [ \ Supelco \ 57310-U \ SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57310u) Quick View [ \ Supelco \ 57439-U \ SPME Overcoated Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57439u) Quick View [ \ Supelco \ 57316-U \ SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57316u) Quick View [ \ Supelco \ 57285-U \ SPME Fiber Assembly Kit](https://www.sigmaaldrich.com/MY/en/product/supelco/57285u) Quick View [ \ Supelco \ 57321-U \ SPME Fiber Assembly Kit](https://www.sigmaaldrich.com/MY/en/product/supelco/57321u) Quick View [ \ Supelco \ 57325-U \ SPME Fiber Assembly Kit](https://www.sigmaaldrich.com/MY/en/product/supelco/57325u) Quick View [ \ Supelco \ 57359-U \ SPME Portable Field Sampler](https://www.sigmaaldrich.com/MY/en/product/supelco/57359u) Quick View [ \ Supelco \ 548550-U \ Smart SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/548550u) Quick View [ \ Supelco \ 548675-U \ Smart SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/548675u) Quick View [ \ Supelco \ 57904-U \ SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57904u) Quick View [ \ Supelco \ 57923-U \ SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57923u) Quick View [ \ Supelco \ 57921-U \ SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57921u) Quick View [ \ Supelco \ 57907-U \ SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/57907u) Quick View [ \ Supelco \ 548575-U \ Smart SPME Fiber Assembly](https://www.sigmaaldrich.com/MY/en/product/supelco/548575u) Quick View * * * ## Featured Categories [](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-chromatography) [Analytical Chromatography](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-chromatography) Discover our premium products for accurate results in HPLC, GC, Karl Fischer titration, & more. From trace analysis to safety equipment, we've got you covered. [Shop Products](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-chromatography) [](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories) [Solid Phase Micro-Extraction (SPME)](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories) Discover advanced SPME solutions: smart fibers, nitinol-core fibers, BioSPME, for efficient solvent-free sample preparation. [Shop Products](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories) [](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) [GC Columns, Accessories & Adsorbents](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) Achieve superior GC performance with our columns, consumables, and accessories. Explore Supelco® offerings. [Shop Products](https://www.sigmaaldrich.com/MY/en/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) * * * Overview Related Articles & Protocols Support SPME is widely used for a variety of applications involving environmental, biological and pharmaceutical samples, foods and beverages, flavors and fragrances, forensics and toxicology and product testing. Typical uses include: - Environmental analyses of water & air samples - Headspace analysis of trace impurities in polymers and solid samples - Part-per-trillion odor analyses - Flavor analyses of food products - Forensic analysis of arson and explosives samples - Toxicology analyses of blood alcohol or drugs in urine and serum ## [](https://www.sigmaaldrich.com)How Does SPME Work? SPME uses a fiber coated with an extraction phase: a liquid (polymer), a solid (sorbent), or a combination of both. The coated fiber is housed in a protective needle and attached to a holder that looks like a syringe. When the fiber is exposed to a sample, the sample’s analytes partition from the sample matrix into the stationary phase until an equilibrium is established. The fiber’s coating extracts compounds from the sample either by absorption (liquid coatings) or adsorption (solid coatings). After a prescribed extraction time, the fiber is removed and inserted directly into a chromatographic instrument, usually [](https://www.sigmaaldrich.com)[gas chromatography (GC)](https://www.sigmaaldrich.com/MY/en/applications/analytical-chemistry/gas-chromatography) or [HPLC](https://www.sigmaaldrich.com/MY/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc), for desorption and analysis. The desorption in GC of analytes is carried out thermally, whereas HPLC uses a solvent for desorption into a liquid-phase. ## [](https://www.sigmaaldrich.com)Advantages of SPME SPME combines analyte sampling, isolation, and enrichment into one, simple step. By controlling the polarity and thickness of the fiber coating, maintaining consistent sampling time, and controlling several other extraction parameters, SPME allows an analyst to ensure highly consistent and quantifiable results from samples, even when analytes are at low concentrations. Other benefits of SPME include: - Solvent-free - Easy to automate - Non-destructive to samples - Applicable for nearly any sample or matrix - Fibers used are reusable and inexpensive - Small fiber size makes them agreeable to field work - Compatible with GC or HPLC instrumentation ## [](https://www.sigmaaldrich.com)SPME Fibers for GC Analysis Conventional SPME is used to extract and concentrate analytes for the purpose of GC analysis. Extraction is carried out either by direct immersion (DI-SPME), where the fiber is directly immersed in the liquid sample, or headspace SPME (HS-SPME), where the fiber is exposed in the vapor phase above a sample. ## [](https://www.sigmaaldrich.com)Supel™ BioSPME for LC-MS Analysis Supel™ BioSPME is a bioanalytical microsampling and sample preparation technique used to quickly and selectively extract a broad range of analytes from biological samples while repelling unwanted macromolecules (e.g., lipids, proteins). Subsequent analysis is usually performed by LC-MS. Supel™ BioSPME operates via direct extraction, involving no sample pretreatment, and provides a non-exhaustive, equilibrium-based extraction. [](https://www.sigmaaldrich.com/documents-search) [Looking for More Specific Information?](https://www.sigmaaldrich.com/documents-search) Visit our document search for data sheets, certificates and technical documentation. [Find Documents](https://www.sigmaaldrich.com/documents-search) ## Related Articles - [Selection Guide for Supelco SPME Fibers](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/selecting-spme-fibers) Recommended fibers based on analyte type and molecular weight; tips for SPME fiber selection for a variety of applications. - [Supel™ BioSPME 96-Pin Devices](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/supel-biospme-96-pin-devices) Supel™ BioSPME 96-Pin devices enhance speed and simplicity for plasma protein binding studies and LCMS analyses. - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) Answers and tips relating to the application of the solid phase micro-extraction technique. - [Rugged SPME Sampling](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) SPME fibers with CAR/PDMS and PDMS/DVB coatings on nitinol core assembly enhance fiber performance for solid phase microextraction. - [Selecting the Appropriate SPME Fiber Coating –Effect of Analyte Molecular Weight and Polarity](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/selecting-the-appropriate) Selecting the Appropriate SPME Fiber Coating –Effect of Analyte Molecular Weight and Polarity - [See All (24)](https://www.sigmaaldrich.com/MY/en/search/facet-search?focus=sitecontent&term=facet-search) ## Related Protocols - [Fast and Reliable Environmental Analysis of Aldehyde and Ketone Air Pollutants](https://www.sigmaaldrich.com/MY/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/fast-and-reliable) Aldehydes and ketones are ubiquitous air pollutants. Along with esters and ethers, they are major components of indoor air pollution and are therefore important for industrial hygiene applications. - [GC Analysis of Pyrazines in Peanut Butter](https://www.sigmaaldrich.com/MY/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-pyrazines-in-peanut-butter) Separation of various pyrazines and sulfur compounds including carbon disulfide and dimethyl disulfide. - [Bisphenol A Analysis in Food by SPME](https://www.sigmaaldrich.com/MY/en/technical-documents/protocol/analytical-chemistry/solid-phase-microextraction/analysis-of-bisphenol-a) Overcoated SPME fiber enables low-level BPA analysis in food via SPME-GC-MS/MS, meeting tighter migration limit regulations. - [Improved Determination of Volatile Organic Compounds in Water by SPME and GC/MS: ISO Standard 17943](https://www.sigmaaldrich.com/MY/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/drinking-water-testing/iso17943) High performance, reliability, and reproducibility of HS-SPME in combination with GC/MS for the determination of VOCs in water was proven in an interlaboratory trial. The new ISO 17943 using HS-SPME is an improvement on existing official methods for this determination in terms of sensitivity and selectivity. - [Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber](https://www.sigmaaldrich.com/MY/en/technical-documents/protocol/analytical-chemistry/solid-phase-microextraction/standard-methods-gc-analysis-of-geosmin-and-slb) Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt - [See All (15)](https://www.sigmaaldrich.com/MY/en/search/facet-search?focus=sitecontent&term=facet-search) ### Find More Articles and Protocols Enter Keywords Search ## How Can We Help In case of any questions, please submit a [customer support request](https://www.sigmaaldrich.com/MY/en/support/customer-support) or talk to our customer service team: Email [[email protected]](mailto:[email protected]) or call +1 (800) 244-1173 ## Additional Support - [Chromatogram Search](https://www.sigmaaldrich.com/chromatogram-search) Use the Chromatogram Search to identify unknown compounds in your sample. - [Calculators & Apps](https://www.sigmaaldrich.com/MY/en/support/calculators-and-apps) Web Toolbox - science research tools and resources for analytical chemistry, life science, chemical synthesis and materials science. - [Customer Support Request](https://www.sigmaaldrich.com/MY/en/support/customer-support) Customer support including help with orders, products, accounts, and website technical issues. - [FAQ](https://maestro.my.site.com/knowledgeportal/s/) Explore our Frequently Asked Questions for answers to commonly asked questions about our products and services. * * * __Featured Articles__ [Selection Guide for Supelco SPME Fibers](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/selecting-spme-fibers) Recommended fibers based on analyte type and molecular weight; tips for SPME fiber selection for a variety of applications. [Supel™ BioSPME 96-Pin Devices](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/supel-biospme-96-pin-devices) Supel™ BioSPME 96-Pin devices enhance speed and simplicity for plasma protein binding studies and LCMS analyses. [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) Answers and tips relating to the application of the solid phase micro-extraction technique. [Rugged SPME Sampling](https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) SPME fibers with CAR/PDMS and PDMS/DVB coatings on nitinol core assembly enhance fiber performance for solid phase microextraction. * * * ## Related Videos Slide 1 of 4 [](https://www.sigmaaldrich.com) [Installing a SPME Fiber](https://www.sigmaaldrich.com) SPME is inexpensive and reusable. Beginners should consider using a manual SPME fiber and holder. The fiber assemblies are color-coded to signify the polymer coating on the fiber, and contain threads to screw into the holder barrel. [![Watch Video](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme/spme-overview-installing-a-spme-fiber-poster.jpg "

SPME Overview: Installing an SPME Fiber

")](https://www.sigmaaldrich.com) [SPME Overview: Installing an SPME Fiber](https://www.sigmaaldrich.com) Solid-phase microextraction (SPME) fibers are easily installed or replaced in the SPME holder.   [![Headspace sampling with SPME is the preferred method to extract volatile organic compounds.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme/headspace-sampling-of-vocs-with-spme-ms-poster.jpg "

SPME Overview: Headspace Sampling of VOCs with SPME

")](https://www.sigmaaldrich.com) [SPME Overview: Headspace Sampling of VOCs with SPME](https://www.sigmaaldrich.com) Headspace sampling with SPME is the preferred method to extract volatile organic compounds. [![The solid-phase microextraction (SPME) solventless extraction technique is based on the equilibrium between the sample and the coating on the SPME fiber.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme/headspace-sampling-of-vocs-with-spme-ms-poster.jpg "

SPME Overview (Manual Sampling): Solventless Sample Preparation

")](https://www.sigmaaldrich.com) [SPME Overview (Manual Sampling): Solventless Sample Preparation](https://www.sigmaaldrich.com) The solid-phase microextraction (SPME) solventless extraction technique is based on the equilibrium between the sample and the coating on the SPME fiber. Top __Sign In To Continue__ To continue reading please sign in or create an account. 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[Sterile & Aseptic Sampling](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/sampling) - [Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing) - [Cell-Based Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/cell-based-vaccine-manufacturing) - [mRNA Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/mrna-vaccines-process-manufacturing) - [pDNA Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/plasmid-dna-vaccines-manufacturing) - [Protein Subunit Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/protein-subunit-vaccine-manufacturing) - [Viral-like Particles Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/viral-like-particles-vaccine-manufacturing) - [Viral Vector Vaccine Manufacturing](https://www.sigmaaldrich.com/MY/en/applications/pharmaceutical-and-biopharmaceutical-manufacturing/vaccine-manufacturing/viral-vector-vaccine-manufacturing) - [Protein Biology](https://www.sigmaaldrich.com/MY/en/applications/protein-biology) - [ELISA](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/elisa) - [Enzyme Activity Assays](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/enzyme-activity-assays) - [Flow Cytometry](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/flow-cytometry) - [Gel Electrophoresis](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/gel-electrophoresis) - [Immunohistochemistry](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/immunohistochemistry) - [Interrogation of Protein Pathways](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/interrogation-protein-pathways) - [Lysis & Protein Extraction](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/lysis-and-protein-extraction) - [Protein & Nucleic Acid Interactions](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/protein-and-nucleic-acid-interactions) - [Protein Concentration & Buffer Exchange](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/protein-concentration-and-buffer-exchange) - [Protein Expression](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/protein-expression) - [Protein Labeling & Modification](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/protein-labeling-and-modification) - [Protein Pulldown](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/protein-pulldown) - [Protein Purification](https://www.sigmaaldrich.com/MY/en/applications/protein-biology/protein-purification) - 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