Merck
HomeApplicationsGenomicsAdvanced Gene Editing

Advanced Gene Editing

Genome editing techniques

Targeted genome editing has rapidly become a vital tool across the entire research continuum from early discovery to therapeutic application. By enabling scientists to selectively disrupt, recover, repress, or activate gene expression, modern gene editing methods allow for unprecedented exploration of genetic mechanisms governing biological processes. Application of genome engineering ranges from curing developmental disorders to developing disease-resistant crops.


Related Technical Articles

  • Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
  • Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.
  • Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
  • In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
  • Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.
  • See All (17)

Related Protocols

Find More Articles and Protocols


Schematic diagram of the CRISPR gene editing system showing the different types of synthetic gRNA for CRISPR.

Schematic diagram of the CRISPR gene editing system showing the different types of synthetic gRNA for CRISPR.

CRISPR – Accurate, Efficient Gene Editing

Gene editing is a specific and targeted change to a DNA sequence and involves the addition, removal or modification of the DNA. The CRISPR-Cas system (evolved in microbes as a defense mechanism) is the basis for a class of gene-editing tools that are enabling advances from health and diagnostics to agriculture and energy. Using CRISPR, researchers have the power to target a specific gene, gene family, or even screen an entire genome.

Sigma-Aldrich® Advanced Genomics offers an industry leading selection of CRISPR-Cas9 proteins to meet your individual research needs. Choose from multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) and several formats (plasmid, lentivirus, lyophilized protein).

CRISPRi and CRISPRa – Powerful Gene Inhibition and Activation

CRISPRi (CRISPR interference) and CRISPRa (CRISPR activation) deliver highly efficient silencing and activation of genes, respectively, without altering the underlying DNA sequence. When employed in large scale LOF (loss-of-function) and GOF (gain-of-function) screens, researchers are able to identify unique, yet functionally related, gene pathways that are often missed with other methods.

Sigma-Aldrich® Advanced Genomics offers a complete suite of optimized CRISPRi and CRISPRa libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.

Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert.


Related Resources

CRISPR Use License Agreement





Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?