HPLC Method Development in One and a Half Days using the Selectivity Concept
Modern HPLC technologies such as UHPLC, monolithic silica or Fused-Core® particles provide a superior separation efficiency and resolution of peaks. But in general resolution is more strongly impacted by selectivity which can be changed most strongly by different stationary bonded phases. This presentation will discuss the choice of RP HPLC column chemistries such as RP-Amide, Phenyl, Pentafluorophenyl (F5) or Cyano that are available to provide alternative selectivities to traditional C18. It will compare their different retention mechanisms and highlight an approach to develop HPLC method within 1.5 days.
Key learnings of this webinar are:
Everybody doing HPLC Method Development and users of HPLC
Dr. Frank Michel
Merck KGaA, Darmstadt, Germany
Analytical & Chromatography Scientific Advisor
Dr. Frank Michel is Analytical & Chromatography Scientific Advisor for Asia-Pacific, training and communicating the technical background of new and innovative analytical techniques, products, and applications. During 20 years in Analytical Chemistry, he focused on HPLC in pharmaceutical analysis, but additionally gained deep experience into other analytical techniques and application areas such as environmental analysis. Dr. Frank Michel is a member of the Separation Science working group in the German Chemist Society (GDCh) and is particularly interested in stationary phase chemistry for both gas and liquid separations.
Session 1:presented July 24, 2018
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