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Sigma-Aldrich

READYBLUE PROTEIN GEL STAIN

Synonym(s):
protein gel stain, protein stain

quality

MQ100

Quality Level

technique(s)

protein staining: suitable

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Quality Level

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General description

ReadyBlue Protein Gel Stain is a rapid and sensitive colloidal Coomassie stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol. No prior fixing step is necessary.
  • Colorimetric or IR fluorescence (800 nm) imaging
  • Reusable up to 3 times
  • No organic solvents or phosphoric acid
  • Compatible with mass spectrometry
  • Room temperature storage—it′s ready when you are

Application

Protein gel stain

Storage and Stability

Store ReadyBlue Protein Gel Stain at room temperature. Protect From Light.

Legal Information

READYBLUE is a trademark of Sigma-Aldrich Co. LLC

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Articles

Sample Preparation & Gel Electrophoresis Troubleshooting

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

Protocols

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie® stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol.

Coating Tissue Culture Plates with Vitronectin

Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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