Lysis-M reagent is compatible with many different applications:
- reporter assays (e.g., β-galactosidase, luciferase, chloramphenicol acetyltransferase)
- immunoassays (e.g., Western blots, ELISAs, RIAs)
- protein assays (e.g., protein kinase A, protein kinase C, and tyrosine kinase)
- protein purification 
Furthermore, the cell lysate is compatible with protein assays such as Coomassie staining and BCA (2′-Benzoyloxycinnamaldehyde) protein assays, and the reagent can be removed by dialysis.Note:
Mini tablets (included in the kit) contain EDTA (ethylenediaminetetraacetic acid). If the protein of interest is to be purified by IMAC (immobilized metal-chelate affinity chromatography), e.g., Poly-His tagged recombinant proteins, EDTA has to be removed (e.g., by dialysis) prior to the chromatography. Alternatively, the cOmplete™
Lysis-M EDTA-free product can be used.