10812846001

Tris hydrochloride

>99% (titration), pH 7.0-9.0, suitable for FISH

• Synonym(s): Trizma^® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• MDL number: MFCD00012590
• PubChem Substance ID: 329749168

Properties

• assay: >99% (titration)
• form: solid
• packaging: pkg of 500 g
• manufacturer/tradename: Roche
• technique(s): FISH: suitable
• pH: 7.0-9.0
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• absorption: <0.02 at 300 nm at 100 mg/mL
• storage temp.: room temp
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

500 g

Features and Benefits

Crystals

Quality

Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable