Documents
11418165001
Roche
Biotin Protein Labeling Kit
sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA
Recommended Products
usage
sufficient for 5 labeling reactions
Quality Level
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
technique(s)
ELISA: suitable
immunoblotting: suitable
storage temp.
2-8°C
Related Categories
1 of 4
This Item | 11685597910 | 68616 | 51146 |
|---|---|---|---|
| packaging kit of 1 (5 components) | packaging pkg of 40 μL | packaging - | packaging - |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH |
| technique(s) ELISA: suitable, immunoblotting: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
General description
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
Application
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.
Packaging
Quality
Preparation Note
- Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
- PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
- Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).
Other Notes
Kit Components Only
- Blocking Reagent (green screw cap)
- Phosphate Buffered Saline (PBS) (blue screw cap)
- D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
- Dimethylsulfoxide (DMSO) (red screw cap)
- Sephadex G-25 Columns
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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