Merck
All Photos(1)

67996

Sigma-Aldrich

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

All Photos(1)
NACRES:
NA.25

grade

for molecular biology

form

solution

quality

50x Concentrate

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

storage temp.

room temp

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
PPB019PPB010M5755
Sigma-Aldrich

Sigma-Aldrich

67996

Tris Acetate-EDTA buffer Concentrate

Bis-Tris Bicine EDTA buffer pH 7.2, pHast Pack™, powder

Sigma-Aldrich

PPB019

Bis-Tris Bicine EDTA buffer

Tris EDTA Buffer, pH 8.0 pHast Pack™, powder, 10X concentrate

Sigma-Aldrich

PPB010

Tris EDTA Buffer, pH 8.0

MOPS-EDTA-Sodium Acetate (MESA) powder for RNA electrophoresis buffers

Sigma-Aldrich

M5755

MOPS-EDTA-Sodium Acetate (MESA)

grade

for molecular biology

grade

-

grade

-

grade

for molecular biology

form

solution

form

powder

form

powder

form

powder

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

-

impurities

-

impurities

-

pH

8.3±0.2 (25 °C)

pH

7.2

pH

-

pH

6.8-7.2(reconstituted per directions)

density

1.09 g/mL at 20 °C

density

-

density

-

density

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Application

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

Components

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service