Merck
All Photos(1)

93283

Sigma-Aldrich

Tris-EDTA buffer solution

BioUltra, for molecular biology, pH 8.0

All Photos(1)
Synonym(s):
TE buffer solution
MDL number:
MFCD00236359
eCl@ss:
32129211
PubChem Substance ID:
329770217
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

form

liquid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

8.0±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.01

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0

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Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T8280

Tris Acetate-EDTA buffer

Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

Sigma-Aldrich

T9650

Tris Acetate-EDTA buffer

Tris-EDTA buffer solution BioUltra, for molecular biology, pH 7.4

Supelco

93302

Tris-EDTA buffer solution

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

product line

BioUltra

product line

BioReagent

product line

BioReagent

product line

BioUltra

form

liquid

form

powder blend

form

solution

form

liquid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

DNase and RNase, none detected

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

8.0±0.2 (25 °C)

pH

8.1-8.5

pH

-

pH

7.4±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

-

cation traces

-

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

Application

Tris-EDTA buffer solution has been used as a component in Tris-EDTA (TE) buffer to resuspend DNA pellets for total DNA extraction from herbage samples and single-stranded carrier DNA extraction from salmon sperm.

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) and its salts are used in a variety of medical and pharmaceutical applications. It is a calcium chelator that also acts as an anticoagulant and a detoxicant in the case of heavy metal poisoning. EDTA is frequently used in analytical chemistry for complexometric titrations and several other applications. It is used as a food additive to bind metal ions due to its low toxicity.
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.

Features and Benefits

  • DNases, RNases, phosphatases, and protease-free
  • Insoluble matter, passes filter test

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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