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A7906

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
NACRES:
NA.27

biological source

bovine

Quality Level

300

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blood typing: suitable
microbiological culture: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT virus, none detected
VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Bovine Serum Albumin heat shock fraction, pH 7, ≥98%

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assay

≥98%

assay

≥96% (agarose gel electrophoresis)

assay

≥99% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

cold ethanol fractionation

purified by

cold ethanol and heatshock fractionation

purified by

heat shock fractionation

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

Application

Bovine serum albumin has been used-
  • as a component of PCR (polymerase chain reaction) mix
  • in the preparation of dissociated cortical neurons
  • during immunofluorescent labeling
  • as a component of Ca2+-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

also commonly purchased with this product

Product No.
Description
Pricing

recommended

Product No.
Description
Pricing

suggested gloves for splash protection

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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