This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.
P0914
Protein standard
Micro Standard, liquid
Synonym(s):
Albumin from bovine serum, protein standard
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5 AMP
RM 289.00
10 AMP
RM 500.00
About This Item
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Product Name
Protein standard, Micro Standard, liquid
biological source
bovine
form
liquid
contains
0.05% sodium azide as preservative
packaging
ampule of 1 mL
concentration
0.98—1.02 mg protein/mL
technique(s)
ELISA: suitable
color
clear colorless
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ALB(280717)
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1 of 4
This Item | 820452 | 820456 | 820453 |
|---|---|---|---|
| biological source bovine | biological source - | biological source - | biological source - |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form liquid | form lyophilized powder, solution (30%) | form lyophilized powder | form lyophilized powder |
| Gene Information bovine ... ALB(280717) | Gene Information - | Gene Information - | Gene Information - |
| packaging ampule of 1 mL | packaging pkg of 1 kg | packaging pkg of 5 kg | packaging pkg of 10 g |
Application
Concentrated solution for all protein assays.
Protein standard has been used:
Protein standard for all protein assays. The sealed ampules provide assurance that the concentration will be correct with every assay.
Protein standard for all protein assays. The sealed ampules provide assurance that the concentration will be correct with every assay.
This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.
Other Notes
1 mg bovine serum albumin/ml in 0.15 M NaCl
Packaging
Sealed ampules
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Alexander Pekarsky et al.
Bioprocess and biosystems engineering, 42(10), 1611-1624 (2019-07-04)
In biotechnological processes, technical failures in the upstream process often lead to batch loss. It is of great interest to investigate the empirical impact of technical failures to understand and mitigate their impact accurately and reduce economic damage. We investigated
Fatma Kermeoğlu et al.
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The aim of this study was to investigate the possible therapeutic impacts of two pineal hormones, melatonin and 5-methoxytryptophol (5-MTX), in a rat model of acute pulpitis by analyzing biochemical and histopathological parameters. This research was done using 32 male
Screening and characterization of a novel esterase from a metagenomic library
Yun-Jung K, et al.
Protein Expression and Purification, 45(2), 315-323 (2006)
Mudalige Don Hiranya Jayasanka Senavirathna et al.
Water environment research : a research publication of the Water Environment Federation, 95(1), e10834-e10834 (2023-01-13)
This study demonstrated the temporal variation of 2-methylisoborneol (2-MIB) and geosmin (GSM) production of two filamentous cyanobacteria species Pseudanabaena galeata (NIES-512; planktonic) and Phormidium ambiguum (NIES-2119; benthic) exposed to high light intensity (950-1000 μmol m-2 s-1 photosynthetically active radiation). The production of
Alexander Pekarsky et al.
Bioprocess and biosystems engineering, 44(6), 1049-1061 (2021-01-26)
Technical failures lead to deviations in process parameters that can exceed studied process boundaries. The impact on cell and target protein is often unknown. However, investigations on common technical failures might yield interesting insights into process and protein robustness. Recently
Articles
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
Protocols
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
To standardize a procedure for the determination of protein by modified Lowry.
Related Content
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
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Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?
1 answer-
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What is the shipping temperature?
1 answer-
The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?
1 answer-
The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).
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