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Sigma-Aldrich

Tris Buffered Saline

pH 8.0, pHast Pack, powder

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Synonym(s):
1X TBS wash buffer, TBS
MDL number:
NACRES:
NA.21

product line

pHast Pack

form

powder

pH

8.0

foreign activity

DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected

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General description

Tris Buffered Saline, pH 8.0 is a commonly used wash buffer that is isotonic and nontoxic and suitable for use in cell culture and electrophoresis applications.

Application

Tris-buffered saline is intended to use for:
  • Sample preparation
  • ELISA
  • Western Blot and other protein applications
  • Cell culture (recommend to sterile filter)
  • Immunohistochemistry
  • Resuspension and sonication of cells

Features and Benefits

  • Ready-to-use buffer – fast and easy to prep
  • No need to measure or adjust pH, simply add water
  • Saves time and minimizes the effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 50mM Tris, 138mM NaCl, 2.7mM KCl, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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