Merck
All Photos(2)

PPB001

Sigma-Aldrich

Tris Buffered Saline

pH 8.0, pHast Pack, powder

Synonym(s):
1X TBS wash buffer, TBS
MDL number:
NACRES:
NA.21

product line

pHast Pack

form

powder

pH

8.0

foreign activity

DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
T5030T5912T6664
Tris Buffered Saline pH 8.0, pHast Pack™, powder

Sigma-Aldrich

PPB001

Tris Buffered Saline

Tris Buffered Saline tablet

Sigma-Aldrich

T5030

Tris Buffered Saline

Sigma-Aldrich

Sigma-Aldrich

T5912

Tris Buffered Saline

Sigma-Aldrich

Sigma-Aldrich

T6664

Tris Buffered Saline

product line

pHast Pack

product line

-

product line

-

product line

-

form

powder

form

tablet

form

solution

form

powder

pH

8.0

pH

-

pH

-

pH

8.0

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

foreign activity

-

foreign activity

-

foreign activity

-

General description

Tris Buffered Saline, pH 8.0 is a commonly used wash buffer that is isotonic and nontoxic and suitable for use in cell culture and electrophoresis applications.

Application

Tris-buffered saline is intended to use for:
  • Sample preparation
  • ELISA
  • Western Blot and other protein applications
  • Cell culture (recommend to sterile filter)
  • Immunohistochemistry
  • Resuspension and sonication of cells

Features and Benefits

  • Ready-to-use buffer – fast and easy to prep
  • No need to measure or adjust pH, simply add water
  • Saves time and minimizes the effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 50mM Tris, 138mM NaCl, 2.7mM KCl, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service