PPB010
Tris EDTA Buffer, pH 8.0
pHast Pack™, powder, 10X concentrate
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Synonym(s):
10X TE buffer
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
7.9—8.1
solubility
soluble, clear, colorless
cation traces
Fe: <10 ppm
foreign activity
DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected
storage temp.
room temp
Related Categories
General description
10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.
Application
TE buffer is intended for use in:
- Nucleic acid gel electrophoresis
- storage and dilution of DNA and RNA
- DNA automated sequencing
- Immunohistochemistry
Features and Benefits
- Fast and easy Ready-to-use buffer
- Minimizes effort - no need to weigh and adjust pH
- Saves time and eliminates exposure to hazardous chemicals
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouch
Reconstitution
Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT RE 2 Inhalation
Target Organs
Respiratory Tract
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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