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Tris Buffered Saline

pH 7.6, pHast Pack, powder

1X TBS wash buffer, TBS
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product line

pHast Pack

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General description

Tris Buffered Saline, or TBS, pH 7.6 is a commonly used wash buffer that is isotonic and non-toxic and ideal for use in cell culture and electrophoresis applications.


Tris-buffered saline can be used as a wash buffer for:
  • Enzyme Linked Immunosorbent assay (ELISA)
  • Western Blot and other protein applications
  • Suitable for use in cell culture (recommend to sterile filter)
  • Immunohistochemistry
  • Sample preparation
  • Resuspension and sonication of cells

Features and Benefits

  • Ready for use, just dissolve in ultrapure water
  • No calculations, measuring, and pH adjustments needed
  • Save time and minimize effort
  • Biological tests: free of DNase, RNase, Protease, and Nickase
  • Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm


Foil pouches


Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl, pH 7.6 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture applications.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids



Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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