PPB012

1.0% Agarose in Tris-Acetate EDTA Buffer

pHast Pack^™, powder

• Synonym(s): 1% Agarose gel, TAE Agarose blend

Properties

• product line: pHast Pack^™
• Quality Level: 100
• form: powder
• pH: 8.1-8.5(range for the Tris Acetate ETDA buffer prior to blending with agarose)

Description

General description

1% Agarose in Tris Acetate EDTA buffer is a powder blend that readily dissolves in boiling water to quickly make an agarose gel for electrophoresis. 1% Agarose gel can separate DNA or RNA fragments that range from 400 – 8,000 bp. Typically used for electrophoresis of larger nucleic acid fragments.

Application

1% Agarose in 1X TAE can be used in:

• DNA Electrophoresis
• Native RNA Electrophoresis

Features and Benefits

• Quick ready-to-pour 1% agarose gel – prepares 20 gels
• No buffer prep or weighing needed
• Save time, and effort, and minimize costs, compared to precast gels
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
• Compatible with TAE pHast pack^™ buffer

Packaging

Foil pouches

Preparation Note

Prepares 250mL for casting several standard-size gels or one large agarose gel. A small electrophoresis apparatus maybe use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel and for optimal results pair with pHast pack 1X TAE, pH 7.3 running buffer.

Reconstitution

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 40 mM Tris acetate, 1 mM EDTA, and 1.0% (w/v) agarose. Contents tested to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3