PPB013

1.0% Agarose in Tris-Borate EDTA Buffer

pHast Pack^™, powder

• Synonym(s): 1% Agarose gel, TAE Agarose blend, TBE Agarose blend

Properties

• product line: pHast Pack^™
• Quality Level: 100
• form: powder
• pH: 8.1-8.5(range for the Tris Borate ETDA buffer prior to blending with agarose)

Description

General description

1% Agarose in Tris Borate EDTA is a powder blend that can be dissolved in boiling water to quickly make a gel for electrophoresis. 1% Agarose gel can separate DNA or RNA fragments between a range of 300 – 7,000 bp and is ideal for smaller fragments <1kB.

Application

1% Agarose in TBE can be used in:

• DNA Electrophoresis
• Native RNA Electrophoresis

Features and Benefits

• Quick ready-to-pour 1% agarose gel
• No buffer preparation or weighing is needed
• Save time and minimize cost and effort
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Packaging

Foil pouches

Preparation Note

Each packet prepares 250mL of molten agarose that is typically used to cast horizontal gels. A small electrophoresis apparatus may use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel. For optimal results pair with pHast pack running buffer 1X TBE, pH 7.3.

Reconstitution

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 89 mM Tris borate, 2 mM EDTA, and 1.0% (w/v) agarose. Certified to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H360FD
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1