27813

Coomassie protein assay reagent

• MDL number: MFCD00163371
• PubChem Substance ID: 57648233
• NACRES: NA.21

Properties

• form: liquid
• Quality Level: 200
• density: ~1.31 g/mL at 20 °C
• storage temp.: 2-8°C
• SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5/C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
• InChI key: RWVGQQGBQSJDQV-UHFFFAOYSA-M

Description

General description

Coomassie protein assay reagent is a protein analysis reagent used for the determination of total protein concentration. The Coomassie dye in the Coomassie (Bradford) reagent binds to protein in the sample leading to an immediate shift in absorption with a concomitant color change of the solution from red to blue. This spectrophotometric measurement of the intensity of this resulting blue color is used to determine the concentration of protein in solutions.

Application

Suitable for the determination of protein according to Bradford. Five-fold concentrate for tests in microtiter plates; ten-fold concentrate for tests in photometric cuvettes (d=1cm); dilute with H₂O

Legal Information

Safety Information

• Pictograms: GHS02,GHS05,GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H226 - H290 - H302 + H312 + H332 - H314 - H370
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 109.4 °F
• Flash Point(C): 43 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves